|
| Status |
Public on Jul 08, 2013 |
| Title |
TNBS colitis day 28_mouse#3 |
| Sample type |
RNA |
| |
|
| Source name |
distal colon
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
disease status: TNBS colitis
time: day 28
animal: mouse#3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Illumnia TotalPrep RNA Amplification Biotinylated cRNA were prepared using Illumnia TotalPrep RNA Amplification Biotinylated cRNA were prepared using Illumnia TotalPrep RNA Amplification
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate _3
|
| Data processing |
Image Analysis and extraction of raw and background subsracted expression data were preformed with Genome Studio software. GeneSpring GX 11.0 was used for quantile normalization and converting expression levels to a log2 scale
|
| |
|
| Submission date |
Feb 07, 2012 |
| Last update date |
Jul 08, 2013 |
| Contact name |
Rob Mariman |
| E-mail(s) |
rob.mariman@tno.nl
|
| Organization name |
TNO
|
| Street address |
Vestwal 5
|
| City |
Leiden |
| ZIP/Postal code |
2312NP |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE35609 |
Analysis of temporal gene expression responses during TNBS-induced colitis development |
|
