|
| Status |
Public on Dec 20, 2012 |
| Title |
YB7 |
| Sample type |
RNA |
| |
|
| Source name |
Immature Sertoli (19 dpc embro testis)
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Immature Sertoli (19 dpc embro testis)
transgene: none
genotype/variation: wild type
|
| Treatment protocol |
The ieSCs were cultured for 6 months, the MEFs and the TTFs were cultured for 3 passages. The immature and mature sertoli cells were cultured for 3 and 7 days respectivly.
|
| Growth protocol |
For ieSCs samples (2%FBS in DME medium and 2mg/ml doxycyclin) for MEFs, TTFS, immature and mature Sertoli cells (10%FBS in DME). The 14.5 dpc male gonad was not cultured at all.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNAEASY kit (Qiagen) following the manufacture's recommendation.
|
| Label |
Cy3
|
| Label protocol |
standard Agilent protocol
|
| |
|
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
standard Agilent protocol
|
| Data processing |
Quantile normalization using limma R package
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
Dec 20, 2012 |
| Contact name |
Albert W Cheng |
| E-mail(s) |
awcheng@mit.edu
|
| Organization name |
Whitehead Institute
|
| Street address |
Room 453, 9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL13912 |
| Series (2) |
| GSE35653 |
Direct reprogramming of fibroblasts into embryonic Sertoli-like cells by defined factors |
| GSE39648 |
Gene expression comparison between different stages of endogenous Sertoli cells and induced embryonic Sertoli cells derived from MEFs and TTFs |
|
