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Sample GSM872972 Query DataSets for GSM872972
Status Public on Dec 20, 2012
Title YB12
Sample type RNA
 
Source name ieSCs (derived fromMEFs)
Organism Mus musculus
Characteristics cell type: ieSCs (derived from MEFs)
transgene: Nr5a1, Wt1, Dmrt1, Gata4 and Sox9
genotype/variation: transdifferentiated cells
Treatment protocol The ieSCs were cultured for 6 months, the MEFs and the TTFs were cultured for 3 passages. The immature and mature sertoli cells were cultured for 3 and 7 days respectivly.
Growth protocol For ieSCs samples (2%FBS in DME medium and 2mg/ml doxycyclin) for MEFs, TTFS, immature and mature Sertoli cells (10%FBS in DME). The 14.5 dpc male gonad was not cultured at all.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNAEASY kit (Qiagen) following the manufacture's recommendation.
Label Cy3
Label protocol standard Agilent protocol
 
Hybridization protocol standard Agilent protocol
Scan protocol standard Agilent protocol
Data processing Quantile normalization using limma R package
 
Submission date Feb 09, 2012
Last update date Dec 20, 2012
Contact name Albert W Cheng
E-mail(s) awcheng@mit.edu
Organization name Whitehead Institute
Street address Room 453, 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL13912
Series (2)
GSE35653 Direct reprogramming of fibroblasts into embryonic Sertoli-like cells by defined factors
GSE39648 Gene expression comparison between different stages of endogenous Sertoli cells and induced embryonic Sertoli cells derived from MEFs and TTFs

Data table header descriptions
ID_REF
VALUE Normalized values in log2

Data table
ID_REF VALUE
1 13.65010805
2 4.086757215
3 4.274548897
4 4.829219421
5 8.238045817
6 6.064216855
7 5.192960336
8 11.20595066
9 4.557670686
10 3.372640438
11 7.693034174
12 4.630235509
13 10.80559101
14 14.60519023
15 6.074094376
16 5.495681806
17 13.25602655
18 8.654006842
19 13.17732727
20 16.03153084

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM872972_252800513175_201201041053_S01_GE1-v5_10_Apr08_1_4.txt.gz 12.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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