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Sample GSM873271 Query DataSets for GSM873271
Status Public on Dec 01, 2012
Title Mouse embryonic fibroblasts wt #2 (biological rep 2)
Sample type RNA
 
Source name Mouse embryonic fibroblasts (MEFs) derived from wt embryo at 13.5 dpc
Organism Mus musculus
Characteristics strain: >98%C57BL6/CBA
genotype: wild type
cell type: mouse embryonic fibroblasts (MEFs)
developmental stage: E13.5
Treatment protocol Mouse embryonic fibroblasts (MEFs) were prepared from embryos of the different genotypes at day 13.5 of gestation
Growth protocol Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 embryos and cultured in DMEM medium supplemented with 10% FBS, penicillin/streptomycin and 2 mM L-glutamine
Extracted molecule total RNA
Extraction protocol Trizol + QIAGEN RNeasy total RNA isolation kit
Label biotin
Label protocol Biotinylated cRNA was prepared from total RNA using the GeneChip IVT Express Kit (Affymetrix).
 
Hybridization protocol Biotinylated cRNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays using the GeneChip Hybridization, Wash and Stain Kit (Affymetrix).
Scan protocol Affymetrix HT scanner with CCD camera
Description MEF_wt #2
Data processing The data was normalised using PLIER algorithm
 
Submission date Feb 09, 2012
Last update date Dec 01, 2012
Contact name Manoj Bhasin
Phone 6176670009
Organization name Beth Israel Deaconess Medical Center
Street address 330 Brookline Avenue RN 380E
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL11180
Series (1)
GSE35670 Systemic elevation of PTEN induces a tumor suppressive metabolic state

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 187.847
AFFX-BioB-M_at 250.087
AFFX-BioB-3_at 226.876
AFFX-BioC-5_at 351.374
AFFX-BioC-3_at 503.836
AFFX-BioDn-5_at 1229.59
AFFX-BioDn-3_at 2204.09
AFFX-CreX-5_at 4488.6
AFFX-CreX-3_at 5479.46
AFFX-DapX-5_at 109.02
AFFX-DapX-M_at 377.054
AFFX-DapX-3_at 683.317
AFFX-LysX-5_at 94.6332
AFFX-LysX-M_at 128.302
AFFX-LysX-3_at 205.948
AFFX-PheX-5_at 106.628
AFFX-PheX-M_at 148.759
AFFX-PheX-3_at 197.257
AFFX-ThrX-5_at 89.8071
AFFX-ThrX-M_at 110.089

Total number of rows: 45141

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM873271_Wt-2_2.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM873271_Wt-2_2.chp.gz 314.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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