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Status |
Public on Dec 01, 2012 |
Title |
Mouse embryonic fibroblasts wt #2 (biological rep 2) |
Sample type |
RNA |
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Source name |
Mouse embryonic fibroblasts (MEFs) derived from wt embryo at 13.5 dpc
|
Organism |
Mus musculus |
Characteristics |
strain: >98%C57BL6/CBA genotype: wild type cell type: mouse embryonic fibroblasts (MEFs) developmental stage: E13.5
|
Treatment protocol |
Mouse embryonic fibroblasts (MEFs) were prepared from embryos of the different genotypes at day 13.5 of gestation
|
Growth protocol |
Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 embryos and cultured in DMEM medium supplemented with 10% FBS, penicillin/streptomycin and 2 mM L-glutamine
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol + QIAGEN RNeasy total RNA isolation kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared from total RNA using the GeneChip IVT Express Kit (Affymetrix).
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|
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Hybridization protocol |
Biotinylated cRNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays using the GeneChip Hybridization, Wash and Stain Kit (Affymetrix).
|
Scan protocol |
Affymetrix HT scanner with CCD camera
|
Description |
MEF_wt #2
|
Data processing |
The data was normalised using PLIER algorithm
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Submission date |
Feb 09, 2012 |
Last update date |
Dec 01, 2012 |
Contact name |
Manoj Bhasin |
Phone |
6176670009
|
Organization name |
Beth Israel Deaconess Medical Center
|
Street address |
330 Brookline Avenue RN 380E
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE35670 |
Systemic elevation of PTEN induces a tumor suppressive metabolic state |
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