|
| Status |
Public on Dec 01, 2012 |
| Title |
Mouse embryonic fibroblasts tg #5 (biological rep 2) |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts (MEFs) derived from tg embryo at 13.5 dpc
|
| Organism |
Mus musculus |
| Characteristics |
strain: >98%C57BL6/CBA
genotype: transgenic
cell type: mouse embryonic fibroblasts (MEFs)
developmental stage: E13.5
|
| Treatment protocol |
Mouse embryonic fibroblasts (MEFs) were prepared from embryos of the different genotypes at day 13.5 of gestation
|
| Growth protocol |
Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 embryos and cultured in DMEM medium supplemented with 10% FBS, penicillin/streptomycin and 2 mM L-glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol + QIAGEN RNeasy total RNA isolation kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from total RNA using the GeneChip IVT Express Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays using the GeneChip Hybridization, Wash and Stain Kit (Affymetrix).
|
| Scan protocol |
Affymetrix HT scanner with CCD camera
|
| Description |
MEF_tg#5
|
| Data processing |
The data was normalised using PLIER algorithm
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Manoj Bhasin |
| Phone |
6176670009
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Street address |
330 Brookline Avenue RN 380E
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE35670 |
Systemic elevation of PTEN induces a tumor suppressive metabolic state |
|
