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Sample GSM87379 Query DataSets for GSM87379
Status Public on May 01, 2006
Title Adult kidney rep 1 (430A)
Sample type RNA
 
Source name Adult kidney replicate 1
Organism Mus musculus
Characteristics Wild type, CD-1 strain, greater than 10 week old (Adult), whole kidney
Extracted molecule total RNA
Extraction protocol Both kidneys were removed from a sacrificed adult mouse, rinsed in PBS, total RNA was prepared using RNAzol (Tel-Test, Friendwood, TX, USA)
Label biotin
Label protocol 100 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type adult complete (whole) kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 657.6 P 0.00762
AFFX-BioB-M_at 873.4 P 0.000081
AFFX-BioB-3_at 425.9 P 0.004017
AFFX-BioC-5_at 1420.8 P 0.00011
AFFX-BioC-3_at 1016.4 P 0.00006
AFFX-BioDn-5_at 1497.8 P 0.00007
AFFX-BioDn-3_at 8876.3 P 0.000195
AFFX-CreX-5_at 10847.6 P 0.000044
AFFX-CreX-3_at 22088 P 0.000044
AFFX-DapX-5_at 29 A 0.425962
AFFX-DapX-M_at 34.6 A 0.382599
AFFX-DapX-3_at 16.4 A 0.876428
AFFX-LysX-5_at 3.9 A 0.921998
AFFX-LysX-M_at 14.6 A 0.852061
AFFX-LysX-3_at 6.8 A 0.834139
AFFX-PheX-5_at 10.4 A 0.904333
AFFX-PheX-M_at 9.7 A 0.852061
AFFX-PheX-3_at 102.1 A 0.425962
AFFX-ThrX-5_at 9.1 A 0.9273
AFFX-ThrX-M_at 14.7 A 0.574038

Total number of rows: 22690

Table truncated, full table size 628 Kbytes.




Supplementary file Size Download File type/resource
GSM87379.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM87379.EXP.gz 497 b (ftp)(http) EXP
Raw data provided as supplementary file

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