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Sample GSM87381 Query DataSets for GSM87381
Status Public on May 01, 2006
Title Hoxa11, Hoxd11 null E12.5 kidney rep 2 (430A)
Sample type RNA
 
Source name Hoxa11, Hoxd11 null E12.5 kidneys replicate 2
Organism Mus musculus
Characteristics Hoxa11, Hoxd11 double null targeted embryonic kidney at E12.5, mixed C57, C3H and CF1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Both kidneys were cleanly dissected from an E12.5 HoxA11/D11 targeted null embryo, rinsed in PBS, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Hoxa11, Hoxd11 double null targeted complete (whole) E12.5 kidney replicate 2
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 388.4 P 0.030976
AFFX-BioB-M_at 366.9 P 0.000857
AFFX-BioB-3_at 157.6 M 0.054213
AFFX-BioC-5_at 856.5 P 0.000581
AFFX-BioC-3_at 502.7 P 0.00034
AFFX-BioDn-5_at 644.7 P 0.000297
AFFX-BioDn-3_at 4957.4 P 0.000297
AFFX-CreX-5_at 7187.5 P 0.000044
AFFX-CreX-3_at 15501 P 0.000044
AFFX-DapX-5_at 55.3 A 0.185131
AFFX-DapX-M_at 65.9 A 0.139482
AFFX-DapX-3_at 54.8 A 0.617401
AFFX-LysX-5_at 7.3 A 0.645547
AFFX-LysX-M_at 12.8 A 0.868639
AFFX-LysX-3_at 47.1 A 0.205732
AFFX-PheX-5_at 14.2 A 0.804734
AFFX-PheX-M_at 4.6 A 0.921998
AFFX-PheX-3_at 106.3 A 0.39692
AFFX-ThrX-5_at 24.8 A 0.686277
AFFX-ThrX-M_at 44.8 A 0.250796

Total number of rows: 22690

Table truncated, full table size 630 Kbytes.




Supplementary file Size Download File type/resource
GSM87381.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM87381.EXP.gz 503 b (ftp)(http) EXP
Raw data provided as supplementary file

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