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Sample GSM87382 Query DataSets for GSM87382
Status Public on May 01, 2006
Title Hoxa11, Hoxd11 null E13.5 kidney rep 1 (430A)
Sample type RNA
 
Source name Hoxa11, Hoxd11 null E13.5 kidneys replicate 1
Organism Mus musculus
Characteristics Hoxa11, Hoxd11 double null targeted embryonic kidney at E13.5, mixed C57, C3H and CF1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Both kidneys were cleanly dissected from an E13.5 HoxA11/D11 targeted null embryo, rinsed in PBS, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Hoxa11, Hoxd11 double null targeted complete (whole) E13.5 kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 414.7 P 0.009337
AFFX-BioB-M_at 528.4 P 0.000225
AFFX-BioB-3_at 244.6 P 0.002023
AFFX-BioC-5_at 845.7 P 0.000225
AFFX-BioC-3_at 553.8 P 0.000052
AFFX-BioDn-5_at 863.1 P 0.000095
AFFX-BioDn-3_at 4830.5 P 0.000195
AFFX-CreX-5_at 8104.7 P 0.000044
AFFX-CreX-3_at 16688.3 P 0.000044
AFFX-DapX-5_at 58.6 A 0.195266
AFFX-DapX-M_at 31.2 A 0.227636
AFFX-DapX-3_at 6.7 A 0.891021
AFFX-LysX-5_at 4.6 A 0.783476
AFFX-LysX-M_at 11 A 0.699394
AFFX-LysX-3_at 18.3 A 0.250796
AFFX-PheX-5_at 13.1 A 0.883887
AFFX-PheX-M_at 6.3 A 0.891021
AFFX-PheX-3_at 80.9 A 0.354453
AFFX-ThrX-5_at 17.6 A 0.712257
AFFX-ThrX-M_at 40.9 A 0.175328

Total number of rows: 22690

Table truncated, full table size 630 Kbytes.




Supplementary file Size Download File type/resource
GSM87382.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM87382.EXP.gz 504 b (ftp)(http) EXP
Raw data provided as supplementary file

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