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Sample GSM87386 Query DataSets for GSM87386
Status Public on May 01, 2006
Title Adult kidney rep 2 (430A)
Sample type RNA
Source name Adult kidney replicate 2
Organism Mus musculus
Characteristics Wild type, CD-1 strain, greater than 10 week old (Adult), whole kidney
Extracted molecule total RNA
Extraction protocol Both kidneys were removed from a sacrificed adult mouse, rinsed in PBS, total RNA was prepared using RNAzol (Tel-Test, Friendwood, TX, USA)
Label biotin
Label protocol 100 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type adult complete (whole) kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 503.5 P 0.00844
AFFX-BioB-M_at 939.5 P 0.000081
AFFX-BioB-3_at 262.4 P 0.002556
AFFX-BioC-5_at 1196 P 0.00011
AFFX-BioC-3_at 964.1 P 0.00006
AFFX-BioDn-5_at 1226.7 P 0.00007
AFFX-BioDn-3_at 7404.9 P 0.000195
AFFX-CreX-5_at 10698 P 0.000044
AFFX-CreX-3_at 19159.6 P 0.000044
AFFX-DapX-5_at 55.8 A 0.116113
AFFX-DapX-M_at 55.7 A 0.440646
AFFX-DapX-3_at 20.4 A 0.712257
AFFX-LysX-5_at 24.9 A 0.51489
AFFX-LysX-M_at 14 A 0.772364
AFFX-LysX-3_at 55.1 A 0.185131
AFFX-PheX-5_at 10 A 0.824672
AFFX-PheX-M_at 5.1 A 0.9273
AFFX-PheX-3_at 135.3 A 0.275146
AFFX-ThrX-5_at 23.1 A 0.876428
AFFX-ThrX-M_at 53.5 A 0.39692

Total number of rows: 22690

Table truncated, full table size 628 Kbytes.

Supplementary file Size Download File type/resource
GSM87386.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM87386.EXP.gz 498 b (ftp)(http) EXP
Raw data provided as supplementary file

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