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Sample GSM87387 Query DataSets for GSM87387
Status Public on May 01, 2006
Title Wild type E12.5 kidney rep 1 (430A)
Sample type RNA
Source name Wild type E12.5 kidneys replicate 1
Organism Mus musculus
Characteristics Wild type embryonic kidney at E12.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E12.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E12.5 kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 443 P 0.004484
AFFX-BioB-M_at 857.5 P 0.000044
AFFX-BioB-3_at 269.4 P 0.000095
AFFX-BioC-5_at 1491.5 P 0.000081
AFFX-BioC-3_at 1094.2 P 0.000044
AFFX-BioDn-5_at 1332.8 P 0.000044
AFFX-BioDn-3_at 6560.9 P 0.00006
AFFX-CreX-5_at 12686.2 P 0.000044
AFFX-CreX-3_at 19127.8 P 0.000044
AFFX-DapX-5_at 41.1 A 0.147939
AFFX-DapX-M_at 62.9 A 0.156732
AFFX-DapX-3_at 7.6 A 0.824672
AFFX-LysX-5_at 11.5 A 0.686277
AFFX-LysX-M_at 10.9 A 0.794268
AFFX-LysX-3_at 21.5 A 0.60308
AFFX-PheX-5_at 8.6 A 0.9273
AFFX-PheX-M_at 5.8 A 0.860518
AFFX-PheX-3_at 53.5 A 0.262827
AFFX-ThrX-5_at 30.7 A 0.672921
AFFX-ThrX-M_at 21.4 A 0.559354

Total number of rows: 22690

Table truncated, full table size 628 Kbytes.

Supplementary file Size Download File type/resource
GSM87387.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM87387.EXP.gz 498 b (ftp)(http) EXP
Raw data provided as supplementary file

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