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Status
Public on May 01, 2006
Title
Wild type E12.5 kidney rep 1 (430A)
Sample type
RNA
Source name
Wild type E12.5 kidneys replicate 1
Organism
Mus musculus Characteristics
Wild type embryonic kidney at E12.5, CD-1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Kidneys were cleanly dissected from an wild type CD-1 E12.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wildtype complete (whole) E12.5 kidney replicate 1
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL339 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
443
P
0.004484
AFFX-BioB-M_at
857.5
P
0.000044
AFFX-BioB-3_at
269.4
P
0.000095
AFFX-BioC-5_at
1491.5
P
0.000081
AFFX-BioC-3_at
1094.2
P
0.000044
AFFX-BioDn-5_at
1332.8
P
0.000044
AFFX-BioDn-3_at
6560.9
P
0.00006
AFFX-CreX-5_at
12686.2
P
0.000044
AFFX-CreX-3_at
19127.8
P
0.000044
AFFX-DapX-5_at
41.1
A
0.147939
AFFX-DapX-M_at
62.9
A
0.156732
AFFX-DapX-3_at
7.6
A
0.824672
AFFX-LysX-5_at
11.5
A
0.686277
AFFX-LysX-M_at
10.9
A
0.794268
AFFX-LysX-3_at
21.5
A
0.60308
AFFX-PheX-5_at
8.6
A
0.9273
AFFX-PheX-M_at
5.8
A
0.860518
AFFX-PheX-3_at
53.5
A
0.262827
AFFX-ThrX-5_at
30.7
A
0.672921
AFFX-ThrX-M_at
21.4
A
0.559354
22690 628 Kbytes .Supplementary file Size Download File type/resource GSM87387.CEL.gz
3.5 Mb
(ftp) (http) CEL
GSM87387.EXP.gz
498 b
(ftp) (http) EXP
