GEO help: Mouse over screen elements for information.
Status
Public on May 01, 2006
Title
Wild type E12.5 kidney rep 2 (430A)
Sample type
RNA
Source name
Wild type E12.5 kidneys replicate 2
Organism
Mus musculus Characteristics
Wild type embryonic kidney at E12.5, CD-1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Kidneys were cleanly dissected from an wild type CD-1 E12.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wildtype complete (whole) E12.5 kidney replicate 2
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL339 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
404.2
P
0.01667
AFFX-BioB-M_at
508.5
P
0.000169
AFFX-BioB-3_at
266.4
P
0.001796
AFFX-BioC-5_at
1240
P
0.00011
AFFX-BioC-3_at
1011.7
P
0.000052
AFFX-BioDn-5_at
1006.4
P
0.000052
AFFX-BioDn-3_at
7214.7
P
0.000127
AFFX-CreX-5_at
11124.9
P
0.000044
AFFX-CreX-3_at
20134.1
P
0.000044
AFFX-DapX-5_at
33.8
A
0.131361
AFFX-DapX-M_at
42.5
A
0.313723
AFFX-DapX-3_at
10
A
0.783476
AFFX-LysX-5_at
4.4
A
0.814869
AFFX-LysX-M_at
33.5
A
0.672921
AFFX-LysX-3_at
18.3
A
0.470238
AFFX-PheX-5_at
9
A
0.916408
AFFX-PheX-M_at
2.4
A
0.973889
AFFX-PheX-3_at
54.6
A
0.313723
AFFX-ThrX-5_at
16.9
A
0.699394
AFFX-ThrX-M_at
52.9
A
0.216524
22690 628 Kbytes .Supplementary file Size Download File type/resource GSM87388.CEL.gz
3.5 Mb
(ftp) (http) CEL
GSM87388.EXP.gz
495 b
(ftp) (http) EXP
