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Sample GSM87389 Query DataSets for GSM87389
Status Public on May 01, 2006
Title Wild type E13.5 kidney rep 1 (430A)
Sample type RNA
Source name Wild type E13.5 kidneys replicate 1
Organism Mus musculus
Characteristics Wild type embryonic kidney at E13.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E13.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E13.5 kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 738.8 P 0.002867
AFFX-BioB-M_at 1297.2 P 0.000052
AFFX-BioB-3_at 605.2 P 0.000446
AFFX-BioC-5_at 2927.9 P 0.00011
AFFX-BioC-3_at 1640.8 P 0.000052
AFFX-BioDn-5_at 2130.2 P 0.000044
AFFX-BioDn-3_at 10546.8 P 0.00006
AFFX-CreX-5_at 21731.8 P 0.000044
AFFX-CreX-3_at 30177 P 0.000044
AFFX-DapX-5_at 31.6 A 0.5
AFFX-DapX-M_at 84.8 A 0.262827
AFFX-DapX-3_at 68 A 0.574038
AFFX-LysX-5_at 8.9 A 0.672921
AFFX-LysX-M_at 20.3 A 0.672921
AFFX-LysX-3_at 4.7 A 0.672921
AFFX-PheX-5_at 4.4 A 0.945787
AFFX-PheX-M_at 14 A 0.749204
AFFX-PheX-3_at 182.1 A 0.078002
AFFX-ThrX-5_at 22 A 0.51489
AFFX-ThrX-M_at 68.9 A 0.275146

Total number of rows: 22690

Table truncated, full table size 629 Kbytes.

Supplementary file Size Download File type/resource
GSM87389.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM87389.EXP.gz 495 b (ftp)(http) EXP
Raw data provided as supplementary file

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