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Status
Public on May 01, 2006
Title
Wild type E13.5 kidney rep 1 (430A)
Sample type
RNA
Source name
Wild type E13.5 kidneys replicate 1
Organism
Mus musculus Characteristics
Wild type embryonic kidney at E13.5, CD-1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Kidneys were cleanly dissected from an wild type CD-1 E13.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wildtype complete (whole) E13.5 kidney replicate 1
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL339 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
738.8
P
0.002867
AFFX-BioB-M_at
1297.2
P
0.000052
AFFX-BioB-3_at
605.2
P
0.000446
AFFX-BioC-5_at
2927.9
P
0.00011
AFFX-BioC-3_at
1640.8
P
0.000052
AFFX-BioDn-5_at
2130.2
P
0.000044
AFFX-BioDn-3_at
10546.8
P
0.00006
AFFX-CreX-5_at
21731.8
P
0.000044
AFFX-CreX-3_at
30177
P
0.000044
AFFX-DapX-5_at
31.6
A
0.5
AFFX-DapX-M_at
84.8
A
0.262827
AFFX-DapX-3_at
68
A
0.574038
AFFX-LysX-5_at
8.9
A
0.672921
AFFX-LysX-M_at
20.3
A
0.672921
AFFX-LysX-3_at
4.7
A
0.672921
AFFX-PheX-5_at
4.4
A
0.945787
AFFX-PheX-M_at
14
A
0.749204
AFFX-PheX-3_at
182.1
A
0.078002
AFFX-ThrX-5_at
22
A
0.51489
AFFX-ThrX-M_at
68.9
A
0.275146
22690 629 Kbytes .Supplementary file Size Download File type/resource GSM87389.CEL.gz
3.4 Mb
(ftp) (http) CEL
GSM87389.EXP.gz
495 b
(ftp) (http) EXP
