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Status
Public on May 01, 2006
Title
Wild type E16.5 kidney rep 2 (430A)
Sample type
RNA
Source name
Wild type E16.5 kidneys replicate 2
Organism
Mus musculus
Characteristics
Wild type embryonic kidney at E16.5, CD-1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Kidneys were cleanly dissected from an wild type CD-1 E16.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wildtype complete (whole) E16.5 kidney replicate 2
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org
Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL339
Series (1)
GSE3808
Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
577.4
P
0.002023
AFFX-BioB-M_at
1097.6
P
0.00007
AFFX-BioB-3_at
420.6
P
0.000297
AFFX-BioC-5_at
1400.6
P
0.00007
AFFX-BioC-3_at
977.5
P
0.000052
AFFX-BioDn-5_at
1507
P
0.000044
AFFX-BioDn-3_at
7605.3
P
0.00007
AFFX-CreX-5_at
15000.2
P
0.000044
AFFX-CreX-3_at
21765.2
P
0.000044
AFFX-DapX-5_at
66
A
0.131361
AFFX-DapX-M_at
71.1
A
0.165861
AFFX-DapX-3_at
46.2
A
0.51489
AFFX-LysX-5_at
5.3
A
0.794268
AFFX-LysX-M_at
14.8
A
0.749204
AFFX-LysX-3_at
22.8
A
0.51489
AFFX-PheX-5_at
10.4
A
0.868639
AFFX-PheX-M_at
3.1
A
0.966323
AFFX-PheX-3_at
53.7
A
0.340661
AFFX-ThrX-5_at
79.7
A
0.262827
AFFX-ThrX-M_at
24
A
0.631562
Total number of rows: 22690 Table truncated, full table size 629 Kbytes .Supplementary file
Size
Download
File type/resource
GSM87392.CEL.gz
3.5 Mb
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CEL
GSM87392.EXP.gz
501 b
(ftp) (http)
EXP