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Sample GSM87392 Query DataSets for GSM87392
Status Public on May 01, 2006
Title Wild type E16.5 kidney rep 2 (430A)
Sample type RNA
 
Source name Wild type E16.5 kidneys replicate 2
Organism Mus musculus
Characteristics Wild type embryonic kidney at E16.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E16.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E16.5 kidney replicate 2
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL339
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 577.4 P 0.002023
AFFX-BioB-M_at 1097.6 P 0.00007
AFFX-BioB-3_at 420.6 P 0.000297
AFFX-BioC-5_at 1400.6 P 0.00007
AFFX-BioC-3_at 977.5 P 0.000052
AFFX-BioDn-5_at 1507 P 0.000044
AFFX-BioDn-3_at 7605.3 P 0.00007
AFFX-CreX-5_at 15000.2 P 0.000044
AFFX-CreX-3_at 21765.2 P 0.000044
AFFX-DapX-5_at 66 A 0.131361
AFFX-DapX-M_at 71.1 A 0.165861
AFFX-DapX-3_at 46.2 A 0.51489
AFFX-LysX-5_at 5.3 A 0.794268
AFFX-LysX-M_at 14.8 A 0.749204
AFFX-LysX-3_at 22.8 A 0.51489
AFFX-PheX-5_at 10.4 A 0.868639
AFFX-PheX-M_at 3.1 A 0.966323
AFFX-PheX-3_at 53.7 A 0.340661
AFFX-ThrX-5_at 79.7 A 0.262827
AFFX-ThrX-M_at 24 A 0.631562

Total number of rows: 22690

Table truncated, full table size 629 Kbytes.




Supplementary file Size Download File type/resource
GSM87392.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM87392.EXP.gz 501 b (ftp)(http) EXP
Raw data provided as supplementary file

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