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Status
Public on May 01, 2006
Title
Hoxa11, Hoxd11 null E12.5 kidney rep 2 (430B)
Sample type
RNA
Source name
Hoxa11, Hoxd11 null E12.5 kidneys replicate 2
Organism
Mus musculus Characteristics
Hoxa11, Hoxd11 double null targeted embryonic kidney at E12.5, mixed C57, C3H and CF1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Both kidneys were cleanly dissected from an E12.5 HoxA11/D11 targeted null embryo, rinsed in PBS, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Hoxa11, Hoxd11 double null targeted complete (whole) E12.5 kidney replicate 2
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL340 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
2176.6
P
0.003212
AFFX-BioB-M_at
3402.2
P
0.000095
AFFX-BioB-3_at
946.6
P
0.001796
AFFX-BioC-5_at
5108.2
P
0.000225
AFFX-BioC-3_at
3558.2
P
0.000044
AFFX-BioDn-5_at
3917.4
P
0.000052
AFFX-BioDn-3_at
24699.4
P
0.000127
AFFX-CreX-5_at
42907.2
P
0.000044
AFFX-CreX-3_at
70889.5
P
0.000044
AFFX-DapX-5_at
110.5
A
0.275146
AFFX-DapX-M_at
218.6
A
0.156732
AFFX-DapX-3_at
69.6
A
0.794268
AFFX-LysX-5_at
14.4
A
0.824672
AFFX-LysX-M_at
53.3
A
0.794268
AFFX-LysX-3_at
159.5
A
0.227636
AFFX-PheX-5_at
35.3
A
0.834139
AFFX-PheX-M_at
25.3
A
0.852061
AFFX-PheX-3_at
123.4
A
0.382599
AFFX-ThrX-5_at
46.2
A
0.672921
AFFX-ThrX-M_at
63.3
A
0.60308
22575 617 Kbytes .Supplementary file Size Download File type/resource GSM87395.CEL.gz
3.4 Mb
(ftp) (http) CEL
GSM87395.EXP.gz
502 b
(ftp) (http) EXP
