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Sample GSM87400 Query DataSets for GSM87400
Status Public on May 01, 2006
Title Adult kidney rep 2 (430B)
Sample type RNA
Source name Adult kidney replicate 2
Organism Mus musculus
Characteristics Wild type, CD-1 strain, greater than 10 week old (Adult), whole kidney
Extracted molecule total RNA
Extraction protocol Both kidneys were removed from a sacrificed adult mouse, rinsed in PBS, total RNA was prepared using RNAzol (Tel-Test, Friendwood, TX, USA)
Label biotin
Label protocol 100 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type adult complete (whole) kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL340
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 2989.1 P 0.006187
AFFX-BioB-M_at 4029.3 P 0.000195
AFFX-BioB-3_at 2093.7 P 0.00141
AFFX-BioC-5_at 7999 P 0.00011
AFFX-BioC-3_at 5656.8 P 0.00011
AFFX-BioDn-5_at 6056.5 P 0.000052
AFFX-BioDn-3_at 44251.7 P 0.000127
AFFX-CreX-5_at 61777.4 P 0.000044
AFFX-CreX-3_at 121086.6 P 0.000044
AFFX-DapX-5_at 130.3 A 0.544587
AFFX-DapX-M_at 331.6 A 0.287743
AFFX-DapX-3_at 250.4 A 0.672921
AFFX-LysX-5_at 25.4 A 0.949771
AFFX-LysX-M_at 106.6 A 0.783476
AFFX-LysX-3_at 61 A 0.470241
AFFX-PheX-5_at 26 A 0.910522
AFFX-PheX-M_at 22.4 A 0.963431
AFFX-PheX-3_at 566.6 A 0.340661
AFFX-ThrX-5_at 120.6 A 0.783476
AFFX-ThrX-M_at 218.8 A 0.58862

Total number of rows: 22575

Table truncated, full table size 618 Kbytes.

Supplementary file Size Download File type/resource
GSM87400.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM87400.EXP.gz 496 b (ftp)(http) EXP
Raw data provided as supplementary file

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