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Sample GSM87400 Query DataSets for GSM87400
Status Public on May 01, 2006
Title Adult kidney rep 2 (430B)
Sample type RNA
 
Source name Adult kidney replicate 2
Organism Mus musculus
Characteristics Wild type, CD-1 strain, greater than 10 week old (Adult), whole kidney
Extracted molecule total RNA
Extraction protocol Both kidneys were removed from a sacrificed adult mouse, rinsed in PBS, total RNA was prepared using RNAzol (Tel-Test, Friendwood, TX, USA)
Label biotin
Label protocol 100 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type adult complete (whole) kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL340
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 2989.1 P 0.006187
AFFX-BioB-M_at 4029.3 P 0.000195
AFFX-BioB-3_at 2093.7 P 0.00141
AFFX-BioC-5_at 7999 P 0.00011
AFFX-BioC-3_at 5656.8 P 0.00011
AFFX-BioDn-5_at 6056.5 P 0.000052
AFFX-BioDn-3_at 44251.7 P 0.000127
AFFX-CreX-5_at 61777.4 P 0.000044
AFFX-CreX-3_at 121086.6 P 0.000044
AFFX-DapX-5_at 130.3 A 0.544587
AFFX-DapX-M_at 331.6 A 0.287743
AFFX-DapX-3_at 250.4 A 0.672921
AFFX-LysX-5_at 25.4 A 0.949771
AFFX-LysX-M_at 106.6 A 0.783476
AFFX-LysX-3_at 61 A 0.470241
AFFX-PheX-5_at 26 A 0.910522
AFFX-PheX-M_at 22.4 A 0.963431
AFFX-PheX-3_at 566.6 A 0.340661
AFFX-ThrX-5_at 120.6 A 0.783476
AFFX-ThrX-M_at 218.8 A 0.58862

Total number of rows: 22575

Table truncated, full table size 618 Kbytes.




Supplementary file Size Download File type/resource
GSM87400.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM87400.EXP.gz 496 b (ftp)(http) EXP
Raw data provided as supplementary file

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