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Sample GSM87400

Query DataSets for GSM87400

Status

Public on May 01, 2006

Title

Adult kidney rep 2 (430B)

Sample type

RNA

Source name

Adult kidney replicate 2

Organism

Mus musculus

Characteristics

Wild type, CD-1 strain, greater than 10 week old (Adult), whole kidney

Extracted molecule

total RNA

Extraction protocol

Both kidneys were removed from a sacrificed adult mouse, rinsed in PBS, total RNA was prepared using RNAzol (Tel-Test, Friendwood, TX, USA)

Label

biotin

Label protocol

100 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

Wild type adult complete (whole) kidney replicate 1

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 12, 2005

Last update date

Dec 12, 2005

Contact name

Kristopher Robert Schwab

E-mail(s)

kristopher.schwab@cchmc.org

Organization name

Cincinnati Children's Hospital Research Foundation

Department

Developmental Biology

Lab

S. Steven Potter

Street address

3333 Burnett Ave

City

Cincinnati

State/province

ZIP/Postal code

45229

Country

USA

Platform ID

GPL340

Series (1)

GSE3808

Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	2989.1	P	0.006187
AFFX-BioB-M_at	4029.3	P	0.000195
AFFX-BioB-3_at	2093.7	P	0.00141
AFFX-BioC-5_at	7999	P	0.00011
AFFX-BioC-3_at	5656.8	P	0.00011
AFFX-BioDn-5_at	6056.5	P	0.000052
AFFX-BioDn-3_at	44251.7	P	0.000127
AFFX-CreX-5_at	61777.4	P	0.000044
AFFX-CreX-3_at	121086.6	P	0.000044
AFFX-DapX-5_at	130.3	A	0.544587
AFFX-DapX-M_at	331.6	A	0.287743
AFFX-DapX-3_at	250.4	A	0.672921
AFFX-LysX-5_at	25.4	A	0.949771
AFFX-LysX-M_at	106.6	A	0.783476
AFFX-LysX-3_at	61	A	0.470241
AFFX-PheX-5_at	26	A	0.910522
AFFX-PheX-M_at	22.4	A	0.963431
AFFX-PheX-3_at	566.6	A	0.340661
AFFX-ThrX-5_at	120.6	A	0.783476
AFFX-ThrX-M_at	218.8	A	0.58862

Total number of rows: 22575

Table truncated, full table size 618 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM87400.CEL.gz	3.3 Mb	(ftp)(http)	CEL
GSM87400.EXP.gz	496 b	(ftp)(http)	EXP