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Status
Public on May 01, 2006
Title
Wild type E12.5 kidney rep 1 (430B)
Sample type
RNA
Source name
Wild type E12.5 kidneys replicate 1
Organism
Mus musculus Characteristics
Wild type embryonic kidney at E12.5, CD-1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Kidneys were cleanly dissected from an wild type CD-1 E12.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wildtype complete (whole) E12.5 kidney replicate 1
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL340 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
3878.2
P
0.004484
AFFX-BioB-M_at
5743
P
0.00007
AFFX-BioB-3_at
2230.6
P
0.001593
AFFX-BioC-5_at
10434.4
P
0.000127
AFFX-BioC-3_at
7914
P
0.00006
AFFX-BioDn-5_at
8872.4
P
0.000044
AFFX-BioDn-3_at
54382.8
P
0.00006
AFFX-CreX-5_at
99674.3
P
0.000044
AFFX-CreX-3_at
161980.5
P
0.000044
AFFX-DapX-5_at
468.1
P
0.039661
AFFX-DapX-M_at
296.4
A
0.250796
AFFX-DapX-3_at
100.5
A
0.659339
AFFX-LysX-5_at
49.4
A
0.814869
AFFX-LysX-M_at
114.3
A
0.772364
AFFX-LysX-3_at
196.8
A
0.313723
AFFX-PheX-5_at
265.4
A
0.659339
AFFX-PheX-M_at
12.6
A
0.966323
AFFX-PheX-3_at
183.1
A
0.39692
AFFX-ThrX-5_at
85.5
A
0.699394
AFFX-ThrX-M_at
71.5
A
0.783476
22575 618 Kbytes .Supplementary file Size Download File type/resource GSM87401.CEL.gz
3.3 Mb
(ftp) (http) CEL
GSM87401.EXP.gz
491 b
(ftp) (http) EXP
