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Sample GSM87402 Query DataSets for GSM87402
Status Public on May 01, 2006
Title Wild type E12.5 kidney rep 2 (430B)
Sample type RNA
 
Source name Wild type E12.5 kidneys replicate 2
Organism Mus musculus
Characteristics Wild type embryonic kidney at E12.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E12.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E12.5 kidney replicate 2
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL340
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 3242.8 P 0.010317
AFFX-BioB-M_at 4317.6 P 0.000081
AFFX-BioB-3_at 1885.7 P 0.003212
AFFX-BioC-5_at 8331.5 P 0.000169
AFFX-BioC-3_at 4875.6 P 0.000044
AFFX-BioDn-5_at 6799.1 P 0.000052
AFFX-BioDn-3_at 43248.8 P 0.000127
AFFX-CreX-5_at 68794.6 P 0.000044
AFFX-CreX-3_at 112657.7 P 0.000044
AFFX-DapX-5_at 460.7 A 0.156732
AFFX-DapX-M_at 473.1 A 0.131361
AFFX-DapX-3_at 67.4 A 0.672921
AFFX-LysX-5_at 18.1 A 0.960339
AFFX-LysX-M_at 140.9 A 0.737173
AFFX-LysX-3_at 81.7 A 0.544587
AFFX-PheX-5_at 61.9 A 0.876428
AFFX-PheX-M_at 30.8 A 0.945787
AFFX-PheX-3_at 361.8 A 0.470241
AFFX-ThrX-5_at 61.3 A 0.904333
AFFX-ThrX-M_at 110.8 A 0.686277

Total number of rows: 22575

Table truncated, full table size 617 Kbytes.




Supplementary file Size Download File type/resource
GSM87402.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM87402.EXP.gz 494 b (ftp)(http) EXP
Raw data provided as supplementary file

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