NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM87403 Query DataSets for GSM87403
Status Public on May 01, 2006
Title Wild type E13.5 kidney rep 1 (430B)
Sample type RNA
 
Source name Wild type E13.5 kidneys replicate 1
Organism Mus musculus
Characteristics Wild type embryonic kidney at E13.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E13.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E13.5 kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
E-mail(s) kristopher.schwab@cchmc.org
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL340
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 3714.9 P 0.006187
AFFX-BioB-M_at 7663.2 P 0.000044
AFFX-BioB-3_at 3722.8 P 0.000147
AFFX-BioC-5_at 15547.1 P 0.000095
AFFX-BioC-3_at 11175.9 P 0.000044
AFFX-BioDn-5_at 12846.3 P 0.000044
AFFX-BioDn-3_at 65270 P 0.00006
AFFX-CreX-5_at 136869.8 P 0.000044
AFFX-CreX-3_at 233985.2 P 0.000044
AFFX-DapX-5_at 55.2 A 0.51489
AFFX-DapX-M_at 473.7 A 0.147939
AFFX-DapX-3_at 101.1 A 0.843268
AFFX-LysX-5_at 39.6 A 0.814869
AFFX-LysX-M_at 79.7 A 0.843268
AFFX-LysX-3_at 305.4 A 0.41138
AFFX-PheX-5_at 76 A 0.945787
AFFX-PheX-M_at 32.3 A 0.963431
AFFX-PheX-3_at 378.8 A 0.368438
AFFX-ThrX-5_at 112.7 A 0.814869
AFFX-ThrX-M_at 77 A 0.772364

Total number of rows: 22575

Table truncated, full table size 619 Kbytes.




Supplementary file Size Download File type/resource
GSM87403.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM87403.EXP.gz 494 b (ftp)(http) EXP
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap