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Status
Public on May 01, 2006
Title
Wild type E13.5 kidney rep 2 (430B)
Sample type
RNA
Source name
Wild type E13.5 kidneys replicate 2
Organism
Mus musculus Characteristics
Wild type embryonic kidney at E13.5, CD-1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Kidneys were cleanly dissected from an wild type CD-1 E13.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wildtype complete (whole) E13.5 kidney replicate 2
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL340 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
7423.4
P
0.00141
AFFX-BioB-M_at
11885.4
P
0.00007
AFFX-BioB-3_at
6504.7
P
0.00006
AFFX-BioC-5_at
19173
P
0.000081
AFFX-BioC-3_at
14052.2
P
0.000044
AFFX-BioDn-5_at
26380.5
P
0.000044
AFFX-BioDn-3_at
77673.8
P
0.000044
AFFX-CreX-5_at
189002.4
P
0.000044
AFFX-CreX-3_at
310375.8
P
0.000044
AFFX-DapX-5_at
754.6
P
0.011384
AFFX-DapX-M_at
364.2
A
0.559354
AFFX-DapX-3_at
425.6
A
0.737173
AFFX-LysX-5_at
49.8
A
0.783476
AFFX-LysX-M_at
150.6
A
0.824672
AFFX-LysX-3_at
69.9
A
0.783476
AFFX-PheX-5_at
71.9
A
0.937071
AFFX-PheX-M_at
65
A
0.860518
AFFX-PheX-3_at
193.6
A
0.699394
AFFX-ThrX-5_at
109.6
A
0.916408
AFFX-ThrX-M_at
102.7
A
0.824672
22575 620 Kbytes .Supplementary file Size Download File type/resource GSM87404.CEL.gz
3.2 Mb
(ftp) (http) CEL
GSM87404.EXP.gz
492 b
(ftp) (http) EXP
