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Sample GSM87405 Query DataSets for GSM87405
Status Public on May 01, 2006
Title Wild type E16.5 kidney rep 1 (430B)
Sample type RNA
Source name Wild type E16.5 kidneys replicate 1
Organism Mus musculus
Characteristics Wild type embryonic kidney at E16.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E16.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E16.5 kidney replicate 1
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL340
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 2402.3 P 0.002023
AFFX-BioB-M_at 4190.5 P 0.000044
AFFX-BioB-3_at 1265 P 0.001593
AFFX-BioC-5_at 6173.9 P 0.00011
AFFX-BioC-3_at 4774.9 P 0.000044
AFFX-BioDn-5_at 5831.1 P 0.000044
AFFX-BioDn-3_at 32203.1 P 0.000081
AFFX-CreX-5_at 63769.8 P 0.000044
AFFX-CreX-3_at 96209.4 P 0.000044
AFFX-DapX-5_at 229.5 A 0.139482
AFFX-DapX-M_at 183.9 A 0.175328
AFFX-DapX-3_at 39.3 A 0.749204
AFFX-LysX-5_at 20.2 A 0.804734
AFFX-LysX-M_at 62.3 A 0.783476
AFFX-LysX-3_at 175.7 A 0.083592
AFFX-PheX-5_at 55.5 A 0.834139
AFFX-PheX-M_at 14.2 A 0.921998
AFFX-PheX-3_at 107.3 A 0.631562
AFFX-ThrX-5_at 65.4 A 0.760937
AFFX-ThrX-M_at 22.4 A 0.749204

Total number of rows: 22575

Table truncated, full table size 618 Kbytes.

Supplementary file Size Download File type/resource
GSM87405.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM87405.EXP.gz 499 b (ftp)(http) EXP
Raw data provided as supplementary file

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