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Sample GSM87406 Query DataSets for GSM87406
Status Public on May 01, 2006
Title Wild type E16.5 kidney rep 2 (430B)
Sample type RNA
Source name Wild type E16.5 kidneys replicate 2
Organism Mus musculus
Characteristics Wild type embryonic kidney at E16.5, CD-1 strain, whole kidney
Extracted molecule total RNA
Extraction protocol Kidneys were cleanly dissected from an wild type CD-1 E16.5 embryo, rinsed in PBS, pooled, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label biotin
Label protocol 30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wildtype complete (whole) E16.5 kidney replicate 2
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 12, 2005
Last update date Dec 12, 2005
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL340
Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 2729.9 P 0.003212
AFFX-BioB-M_at 5747.7 P 0.000044
AFFX-BioB-3_at 2157.7 P 0.00034
AFFX-BioC-5_at 8345 P 0.000095
AFFX-BioC-3_at 5530 P 0.000052
AFFX-BioDn-5_at 7309.9 P 0.000044
AFFX-BioDn-3_at 38787.4 P 0.00006
AFFX-CreX-5_at 83469.7 P 0.000044
AFFX-CreX-3_at 118931.2 P 0.000044
AFFX-DapX-5_at 206.3 A 0.313723
AFFX-DapX-M_at 208.6 A 0.382599
AFFX-DapX-3_at 63.3 A 0.60308
AFFX-LysX-5_at 63.1 A 0.749204
AFFX-LysX-M_at 39.7 A 0.953518
AFFX-LysX-3_at 244.6 A 0.102165
AFFX-PheX-5_at 38.9 A 0.916408
AFFX-PheX-M_at 11.9 A 0.941556
AFFX-PheX-3_at 133.3 A 0.48511
AFFX-ThrX-5_at 53.6 A 0.794268
AFFX-ThrX-M_at 41 A 0.772364

Total number of rows: 22575

Table truncated, full table size 618 Kbytes.

Supplementary file Size Download File type/resource
GSM87406.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM87406.EXP.gz 500 b (ftp)(http) EXP
Raw data provided as supplementary file

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