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Sample GSM874540 Query DataSets for GSM874540
Status Public on Feb 04, 2013
Title 105_after fructose load_rep1
Sample type RNA
 
Source name after 7 days of fructose load
Organism Homo sapiens
Characteristics diet group: PP
tissue: skeletal muscle
sample_id: 105C
Extracted molecule total RNA
Extraction protocol RNA was prepared using the mirVanaTM miRNA Isolation Kit (Applied Biosystems) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 µg RNA using the One-Color Low Input Quick Amp labeling (Agilent) according to the manufacturer's instructions, followed by purification with Absolutely RNA Nanoprep kit (Stratagen). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol 1.65 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 55µl containing 2x Agilent fragmentation buffer and 10x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 55µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4845A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides.
Description Gene Expression after 7 days of fructose load
Data processing The scanned images were analyzed with Feature Extraction Software 10.5 (Agilent) using default parameters to obtain background subtracted median normalized Signal intensities.
 
Submission date Feb 13, 2012
Last update date Feb 04, 2013
Contact name emmanuelle meugnier
E-mail(s) meugnier@univ-lyon1.fr
Organization name INSERMU1060/INRA1397
Lab CarMeN
Street address Cens Eli Bat 2 D- Hopital Lyon Sud
City Pierre Bénite
ZIP/Postal code 69495
Country France
 
Platform ID GPL13497
Series (1)
GSE35764 Polyphenols protection against fructose overload

Data table header descriptions
ID_REF
VALUE log2 Median-normalized signal intensities

Data table
ID_REF VALUE
GE_BrightCorner 8.114222
DarkCorner -7.333855
A_23_P146146 -7.176877
A_23_P42935 -2.4411383
A_23_P117082 2.7915049
A_23_P2683 -0.004418373
A_24_P358131 -6.000779
A_33_P3367647 -7.181213
A_23_P157316 -3.40659
A_32_P14850 7.485977
A_23_P158596 -2.1143384
A_23_P350107 -0.13344955
A_23_P388190 -0.90204525
A_23_P106544 2.9563646
A_33_P3219745 -7.2117033
A_32_P85539 0.8290968
A_23_P94998 -1.4203596
A_33_P3235677 -4.932254
A_23_P417014 -7.2367926
A_23_P103905 1.8154669

Total number of rows: 34183

Table truncated, full table size 793 Kbytes.




Supplementary file Size Download File type/resource
GSM874540_US45103047_252665213173_S01_GE1_107_Sep09_1_2.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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