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Sample GSM876851 Query DataSets for GSM876851
Status Public on Aug 01, 2012
Title C2_white_matter_HIV infected_w/HAD_w/o HIVE
Sample type RNA
 
Source name brain_white_matter
Organism Homo sapiens
Characteristics race: White
age: 35
gender: male
phenotype: HIV Infected with neurocognitive impairment (HAD: HIV-associated dementia)
tissue: White matter
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol After fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Human U133 2.0 plus GeneChips were washed and stained.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000 7G.
Description 14G756.CEL
Gene expression data from the white matter of HIV infected HAD human brain
Data processing The data were analyzed with Affy package for R programming.
 
Submission date Feb 16, 2012
Last update date Aug 01, 2012
Contact name Jessica Winkler
E-mail(s) jwinkle@unmc.edu
Organization name University of Nebraska Medical Center
Department Pharmacology and Experimental Neuroscience
Lab Howard Fox
Street address 985800 Nebraska Medical Center- DRC I
City Omaha
ZIP/Postal code 68198
Country USA
 
Platform ID GPL570
Series (1)
GSE35864 The National NeuroAIDS Tissue Consortium Brain Gene Array: Two types of HIV-associated neurocognitive impairment

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.8561619
1053_at 5.655355725
117_at 6.390812448
121_at 9.121005927
1255_g_at 4.420472031
1294_at 6.568908496
1316_at 7.183023968
1320_at 4.993630978
1405_i_at 4.820519625
1431_at 4.891091881
1438_at 6.427284354
1487_at 7.431733123
1494_f_at 6.466225089
1552256_a_at 9.132376568
1552257_a_at 7.236381773
1552258_at 6.319938235
1552261_at 5.073873453
1552263_at 5.444215411
1552264_a_at 5.467793018
1552266_at 5.013845249

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM876851_14G756.CEL.gz 5.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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