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Sample GSM876881 Query DataSets for GSM876881
Status Public on Aug 01, 2012
Title D1_Frontal_Cortex_HIV infected_w/HAD_w/ HIVE
Sample type RNA
 
Source name brain_frontal_cortex
Organism Homo sapiens
Characteristics race: White
age: 48
gender: male
phenotype: HIV Infected with HAD and HIV encephalitis (HIVE)
tissue: Frontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol After fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Human U133 2.0 plus GeneChips were washed and stained.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000 7G.
Description 44G757.CEL
Gene expression data from the white matter of HIV infected HAD and HIVE human brain
Data processing The data were analyzed with Affy package for R programming.
 
Submission date Feb 16, 2012
Last update date Aug 01, 2012
Contact name Jessica Winkler
E-mail(s) jwinkle@unmc.edu
Organization name University of Nebraska Medical Center
Department Pharmacology and Experimental Neuroscience
Lab Howard Fox
Street address 985800 Nebraska Medical Center- DRC I
City Omaha
ZIP/Postal code 68198
Country USA
 
Platform ID GPL570
Series (1)
GSE35864 The National NeuroAIDS Tissue Consortium Brain Gene Array: Two types of HIV-associated neurocognitive impairment

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 12.5562596
1053_at 7.145093213
117_at 6.949855117
121_at 9.714121989
1255_g_at 5.29794514
1294_at 7.849624181
1316_at 7.781634594
1320_at 6.100844069
1405_i_at 5.298067643
1431_at 5.899416109
1438_at 7.45046895
1487_at 7.915888875
1494_f_at 7.492615768
1552256_a_at 9.365792929
1552257_a_at 8.25871811
1552258_at 6.65963093
1552261_at 6.339767318
1552263_at 5.719286284
1552264_a_at 6.465355496
1552266_at 6.244794005

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM876881_44G757.CEL.gz 5.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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