|
| Status |
Public on Feb 18, 2012 |
| Title |
Adult repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse beta-cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: Pdx1-GFP tg x ICR
age: 5 weeks
tissue: Islets of Langerhans
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Islets were isolated from heterozygous Pdx1–GFP (crossed with ICR) animals and further dissociated into single cells with 0. 25% Trypsin-EDTA (Invitrogen). GFP+ cells were isolated using FACSaria (BD Bioscience). Total RNA was extracted with RNeasy RNA extraction kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from ≥ 100 ng of isolated RNA using Illumina TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
rank invariant
|
| |
|
| Submission date |
Feb 17, 2012 |
| Last update date |
Feb 18, 2012 |
| Contact name |
Doug Melton |
| Organization name |
Harvard University
|
| Department |
Stem Cell and regenerative Biology
|
| Lab |
Prof. Douglas Melton
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE35906 |
Analysis of differential gene expression during functional beta-cell maturation in the mouse |
|
