|
Status |
Public on Feb 18, 2012 |
Title |
Adult repeat 2 |
Sample type |
RNA |
|
|
Source name |
Mouse beta-cells
|
Organism |
Mus musculus |
Characteristics |
strain: Pdx1-GFP tg x ICR age: 5 weeks tissue: Islets of Langerhans
|
Extracted molecule |
total RNA |
Extraction protocol |
Islets were isolated from heterozygous Pdx1–GFP (crossed with ICR) animals and further dissociated into single cells with 0. 25% Trypsin-EDTA (Invitrogen). GFP+ cells were isolated using FACSaria (BD Bioscience). Total RNA was extracted with RNeasy RNA extraction kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared from ≥ 100 ng of isolated RNA using Illumina TotalPrep RNA Amplification Kit (Ambion)
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2
|
Data processing |
rank invariant
|
|
|
Submission date |
Feb 17, 2012 |
Last update date |
Feb 18, 2012 |
Contact name |
Doug Melton |
Organization name |
Harvard University
|
Department |
Stem Cell and regenerative Biology
|
Lab |
Prof. Douglas Melton
|
Street address |
7 Divinity Ave
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE35906 |
Analysis of differential gene expression during functional beta-cell maturation in the mouse |
|