|
| Status |
Public on May 23, 2012 |
| Title |
P12_washout-GSI_24h |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human T-ALL cell line P12 Ichikawa, GSI 4 days, washout, GSI 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human T-ALL cell line P12 Ichikawa
protocol: GSI 24hr
|
| Treatment protocol |
Cells were treated with the gamma-secretase inhibitor (GSI) Compound E (E) at a concentration of 1uM for 4 days followed by washout and resuspension in media containing E or Mock (DMSO).
|
| Growth protocol |
Jurkat and P12 Ichikawa were grown under standard conditions. RPMI 1640 medium supplemented with 10% FCS, 1 mM sodium pyruvate, 2 mM L-glutamine, and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using standard Trizol protocol.
|
| Label |
Hy3
|
| Label protocol |
Individual samples were labelled with Hy3 and compared to a common reference pool of all 4 sampled labelled with Hy5.
|
| |
|
| Channel 2 |
| Source name |
Reference pool (samples R82 J_M, R83 J_E, R85 P12_M, R86 P12_E pooled together)
|
| Organism |
Homo sapiens |
| Characteristics |
reference composition: samples R82 J_M, R83 J_E, R85 P12_M, R86 P12_E pooled together
|
| Treatment protocol |
Cells were treated with the gamma-secretase inhibitor (GSI) Compound E (E) at a concentration of 1uM for 4 days followed by washout and resuspension in media containing E or Mock (DMSO).
|
| Growth protocol |
Jurkat and P12 Ichikawa were grown under standard conditions. RPMI 1640 medium supplemented with 10% FCS, 1 mM sodium pyruvate, 2 mM L-glutamine, and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using standard Trizol protocol.
|
| Label |
Hy5
|
| Label protocol |
Individual samples were labelled with Hy3 and compared to a common reference pool of all 4 sampled labelled with Hy5.
|
| |
|
| |
| Hybridization protocol |
Performed as per Exiqon standard procedures.
|
| Scan protocol |
Performed as per Exiqon standard procedures.
|
| Description |
raw data file ch1: 1_Exiqon_14069971_S01_Cropped.txt
raw data file ch2: 0_Exiqon_14069971_S01_Cropped.txt
Sample name: R86 P12_E
|
| Data processing |
Performed as per Exiqon standard procedures.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
May 23, 2012 |
| Contact name |
Andrew P Weng |
| E-mail(s) |
aweng@bccrc.ca
|
| Phone |
604-675-8136
|
| Fax |
604-877-0712
|
| Organization name |
BC Cancer Agency
|
| Department |
Pathology
|
| Lab |
Terry Fox Laboratory
|
| Street address |
675 West 10th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE35993 |
Notch regulated miRNAs in human T-ALL cell line |
|
