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Sample GSM879117 Query DataSets for GSM879117
Status Public on Nov 01, 2012
Title D1 11:00 replicate 4b_pre EMF exposure
Sample type RNA
 
Source name immediately prior to ELF-EMF exposure
Organism Homo sapiens
Characteristics tissue source: Healthy volunteers
gender: male
age: 20-30 years
tissue: Peripheral blood
study day: Day1
time-point: 11:00
treatment: immediately prior to ELF-EMF exposure
Treatment protocol 17 healthy male subjects age 20 -30 each received an extremely low frequency electromagnetic field exposure (ELF-EMF) on study day 1 repeated on study day 3 (7 days later), a sham exposure ( using counterwound coils in the 1 M cube exposure frames) on study day 2 (the day after study day 1) and a null exposure (no current through coils) on study day 4 (the day after study day 3). All exposures (ELF-EMF, sham or null) were for 2 h at 11.00 - 13.00. 10 ml blood samples were collected at each time point by cannula at 9.00, 11.00, 11.05, 11.10, 11.20,11.40, 12.20, 13.00, 15.00 and 17.00) and transfered directly into PAXgene blood tubes.
Extracted molecule total RNA
Extraction protocol The whole blood samples collected in PAXgene blood tubes were stored a 4C overnight and the RNA was extracted the next day with the PAXgene Blood RNA kit from QIAGEN according to the manufacturer's instructions, including the DNase treatment. A pooling strategy was used to detect significant responses that occur in most or all of the volunteers. Equal amounts of RNA (300 ng/sample) were pooled from the 17 RNA samples samples for each of the 40 time points on study days 1 to 4. The 260/280 nm ratio of all pools was > 2.0. The 40 pooled RNA samples were assayed by Agilent Bioanalyser for RNA Integrity Number (RIN) and all RIN values were >8.0 (38/40>9.0).
Label biotin
Label protocol Biotinylated cRNA samples were prepared with the Illumina TotalPrep RNA Amplification kit from Ambion.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using the Beadarray reader.
Description SAMPLE 47
technical replicate-labelled extract (reference sample)
Data processing Beadstudio v.2.3.47 was used for processing the raw data and the Average method was used for normalization. Averaged data were filtered for probes with detection pvalue < 0.01 in at least one sample.
 
Submission date Feb 22, 2012
Last update date Nov 01, 2012
Contact name James Metcalfe
E-mail(s) jcm@mole.bio.cam.ac.uk
Organization name University of Cambridge
Department Biochemistry
Street address 80 Tennis Court Road
City Cambridge
ZIP/Postal code CB2 8PJ
Country United Kingdom
 
Platform ID GPL6097
Series (1)
GSE35999 Gene expression profiles in white blood cells of volunteers exposed to a 50 Hz electromagnetic field

Data table header descriptions
ID_REF
VALUE Average normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
940746 105.2416 0
940731 1521.943 0
940707 592.7781 0
940692 119.9345 0
940673 35.20366 0.001977587
940671 641.0002 0
940632 249.5338 0
940592 139.2075 0
940577 120.3405 0
940576 25.95452 0.007251154
940546 74.97691 0
940504 335.8689 0
940500 604.6591 0
940494 122.5947 0
940487 928.7302 0
940463 1760.912 0
940452 188.8147 0
940451 179.8325 0
940435 64.97996 0.000659196
940433 1168.088 0

Total number of rows: 13372

Table truncated, full table size 310 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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