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Sample GSM88009 Query DataSets for GSM88009
Status Public on Nov 09, 2006
Title PriMk_Expt1_Day4_rep2
Sample type RNA
 
Channel 1
Source name PriMk_Expt1_Day4
Organism Homo sapiens
Characteristics Culture initiated from G-CSF mobilized peripheral blood CD34-positive hematopoietic stem and progenitor cell donation, male donor, age 24, Caucasian
Biomaterial provider All Cells, Berkeley, CA
Treatment protocol Cultured for 4 days
Growth protocol Cultured as described (Yang et al., Stem Cells 2002;20:320-328) with TPO, IL-3, and Flt3-ligand in serum free X-VIVO media
Extracted molecule total RNA
Extraction protocol Agilent RNA isolation mini-kit
Label Cy3
Label protocol Agilent Low RNA Input Fluorescent Linear Amplification kit (v2.0) (reverse transcription with oligo-dT primers, in vitro transcription with direct incorporation of fluorescently labeled CTP analogs)
 
Channel 2
Source name Universal Reference RNA
Organism Homo sapiens
Characteristics Pooled RNA from 10 human cell lines
Biomaterial provider Stratagene
Extracted molecule total RNA
Label Cy5
Label protocol Agilent Low RNA Input Fluorescent Linear Amplification kit (v2.0) (reverse transcription with oligo-dT primers, in vitro transcription with direct incorporation of fluorescently labeled CTP analogs)
 
 
Hybridization protocol Followed Agilent 60-mer oligo microarray processing protocol v 4.1, using SSC wash protocol and forced nitrogen drying
Scan protocol Scanned using Agilent Microarray Scanner (G2565BA) at 10um resolution
Description Study of in vitro megakaryocyte differentiation
Data processing Agilent Feature Extraction software (v.7.2) was used to assess spot quality and extract feature intensity statistics; duplicate spots were averaged; background-subtracted data were normalized using SNNLERM (Yang et al., PNAS 2003; 100:1122-1127)
 
Submission date Dec 15, 2005
Last update date Aug 24, 2007
Contact name Eleftherios Terry Papoutsakis
E-mail(s) papoutsakis@dbi.udel.edu
Organization name University of Delaware
Department Chemical Engineering
Street address 15 Innovation Way
City Newark
State/province DE
ZIP/Postal code 19711
Country USA
 
Platform ID GPL887
Series (2)
GSE3839 Temporal expression profile of megakaryocytic differentiation primary CD34+ cell culture
GSE8914 CHRF-288-11 and primary human megakaryocytic cell cultures provide novel insights into lineage-specific differentiation

Data table header descriptions
ID_REF
VALUE -[INV_VALUE]
CH1_MEDIAN_INT Channel 1 median intensity
CH1_MEDIAN_BKG Channel 1 median background intensity
CH1_BKG_STD_DEV Channel 1 background intensity standard deviation
CH2_MEDIAN_INT Channel 2 median intensity
CH2_MEDIAN_BKG Channel 2 median background intensity
CH2_BKG_STD_DEV Channel 2 background intensity standard deviation
CONF_DIFF_EXPR Confidence of differential expression (Ch1 vs Ch2, per method of Yang et al., PNAS 2003; 100:1122-1127 [0,1]
INV_VALUE Normalized natural log (Ch2/Ch1). Replicate spots on array representing same 60mer were averaged prior to normalization.

Data table
ID_REF VALUE CH1_MEDIAN_INT CH1_MEDIAN_BKG CH1_BKG_STD_DEV CH2_MEDIAN_INT CH2_MEDIAN_BKG CH2_BKG_STD_DEV CONF_DIFF_EXPR INV_VALUE
1 NULL 1875 37 3.76 221 41 4.56 NULL NULL
2 NULL 105 37 3.83 61 42 4.90 NULL NULL
3 0.7418 143 37 3.87 94 43 4.89 0.8805 -0.7418
4 -0.5535 1414 37 3.56 3877 43 5.15 0.6737 0.5535
5 0.1325 245 37 3.71 307 42 5.01 0.1577 -0.1325
6 NULL 118 37 3.56 75 42 4.79 NULL NULL
7 NULL 1742 37 3.85 86 42 5.13 NULL NULL
8 -0.4352 370 37 3.91 722 43 4.91 0.7299 0.4352
9 0.3254 290 37 3.83 283 43 5.25 0.3936 -0.3254
10 -0.131 103 36 3.84 78 42 4.94 0.6319 0.1310
11 -0.22 115 37 3.88 94 42 5.42 0.2477 0.2200
12 -0.2987 129 37 3.87 120 42 5.11 0.4693 0.2987
13 -0.3073 109 38 3.89 87 43 4.91 0.3406 0.3073
14 NULL 1820 39 3.67 85 42 4.72 NULL NULL
15 -0.5495 606 38 4.26 1351 43 4.54 0.5508 0.5495
16 -0.2964 161 38 3.94 174 43 5.26 0.6320 0.2964
17 NULL 106 38 4.07 69 42 5.05 NULL NULL
18 0.0612 196 38 4.02 231 42 5.43 0.0732 -0.0612
20 0.9061 116 38 3.67 63 42 4.64 1.0000 -0.9061
21 NULL 1920 39 4.01 92 43 4.30 NULL NULL

Total number of rows: 22153

Table truncated, full table size 1092 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
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