|
Status |
Public on Jan 01, 2006 |
Title |
wild type GFP negative rep 1A |
Sample type |
RNA |
|
|
Source name |
nonmesodermal cells
|
Organism |
Drosophila melanogaster |
Characteristics |
These are wild-type embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells. Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11). GFP-negative cells were collected using FACS.
|
Extracted molecule |
total RNA |
Extraction protocol |
The embryos were dounce homogenized into a single cell suspension. These cells were then FACS sorted. The GFP-negative cells from this sort comprise this sample.
|
Label |
biotin
|
Label protocol |
Approximately 2.5-3 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
These are wild-type embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells. Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11). GFP-negative cells were collected using FACS.
|
Data processing |
Bioconductors' affy package in conjunction with Choe et al.'s goldenspike package (Choe et al. Genome Biol. 2005).
|
|
|
Submission date |
Dec 16, 2005 |
Last update date |
Feb 18, 2006 |
Contact name |
Sung Eun Choe |
Organization name |
Wyeth Research
|
Street address |
258 Harvard St #164
|
City |
Brookline |
State/province |
MA |
ZIP/Postal code |
02446 |
Country |
USA |
|
|
Platform ID |
GPL72 |
Series (1) |
GSE3854 |
An integrated strategy for analyzing the unique developmental program of different myoblast subtypes |
|