|
| Status |
Public on Jan 01, 2006 |
| Title |
Ras gain of function rep 2B |
| Sample type |
RNA |
| |
|
| Source name |
mesodermal cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Ras1^{Act} insertion that yields constitutively activated Ras within these Twist expressing cells. Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11). GFP-positive cells were collected using FACS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The embryos were dounce homogenized into a single cell suspension. These cells were then FACS sorted. The GFP-positive cells from this sort comprise this sample.
|
| Label |
biotin
|
| Label protocol |
Approximately 2.5-3 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Ras1^{Act} insertion that yields constitutively activated Ras within these Twist expressing cells. Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11). GFP-positive cells were collected using FACS.
|
| Data processing |
Bioconductors' affy package in conjunction with Choe et al.'s goldenspike package (Choe et al. Genome Biol. 2005).
|
| |
|
| Submission date |
Dec 16, 2005 |
| Last update date |
Feb 18, 2006 |
| Contact name |
Sung Eun Choe |
| Organization name |
Wyeth Research
|
| Street address |
258 Harvard St #164
|
| City |
Brookline |
| State/province |
MA |
| ZIP/Postal code |
02446 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE3854 |
An integrated strategy for analyzing the unique developmental program of different myoblast subtypes |
|
