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Sample GSM883358 Query DataSets for GSM883358
Status Public on Feb 06, 2013
Title JQ1_2h_RNAseq
Sample type SRA
 
Source name S2-DRSC cells, JQ1, 2h
Organism Drosophila melanogaster
Characteristics cell line: S2-DRSC
treatment: JQ1
treatment duration: 2 hrs
Treatment protocol Cells were treated either with JQ1 or DMSO by adding the compound to the growth medium for the indicated time periods.
Growth protocol S2-DRSC cells were grown in Schneider's medium incl. 10% FCS.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using standard Trizol protocols for adherent cells. 4 microg of total RNA served as the starting material to construct the libraries using the TruSeq RNA Sample Prep Kit (Illumina) according to the manufacturer's instructions.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description BSSE-DSU-1736
Data processing Reads were aligned to the Drosophila genome release BDGP5 using the splice junction aware aligner TopHat (http://tophat.cbcb.umd.edu/) and standard parameters. Reads uniquely mapping to Drosophila gene sets (Ensembl release 64) were counted using the htseq-count script (http://www-huber.embl.de/users/anders/HTSeq/doc/count.html#count). Gene sets in GTF format were obtained from http://www.ensembl.org/info/data/ftp/index.html.
Genome Build:
1736.counts: BDGP5
 
Submission date Feb 29, 2012
Last update date May 15, 2019
Contact name Tobias Kockmann
E-mail(s) tobias.kockmann@bsse.ethz.ch
Organization name ETHZ
Department D-BSSE
Lab Paro
Street address Mattenstr. 26
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platform ID GPL13304
Series (2)
GSE36180 Effects of pharmacologic FSH inhibition on gene expression
GSE36450 The BET protein FSH functionally interacts with ASH1 to orchestrate global gene activity in Drosophila
Relations
Reanalyzed by GSM3276692
SRA SRX124541
BioSample SAMN00793126

Supplementary file Size Download File type/resource
GSM883358_1736.counts.txt.gz 58.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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