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Sample GSM883428 Query DataSets for GSM883428
Status Public on Mar 02, 2012
Title DC ko 0h unstim Rep2
Sample type RNA
 
Source name G9a -/- DC
Organism Mus musculus
Characteristics strain: C57Bl/6
genotype: G9a -/-, Vav-cre
cell type: ex vivo isolated splenic DCs
Treatment protocol DCs were directly stimulated with 10 μg/ml polyI:C in RPMI supplemented with 10% FBS, L-Glutamine, Penicillin-Streptomycin, Sodium Pyruvate, HEPES and 2-mercaptoethanol.
Growth protocol To induce expansion of splenic DCs, C57Bl/6, G9afl/fl mice and G9afl/fl;Vav-Cre mice were subcutaneously injected with 5 x 106 B16-Flt3L cells. Two weeks later splenic DCs were enriched for the CD11c+ population using CD11c MACS beads (Miltenyi).
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp Premier RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description G9a -/- DC
Data processing The data were normalised using percentile normalisation with GeneSpringGX 10
 
Submission date Mar 01, 2012
Last update date Mar 02, 2012
Contact name Terry Fang
E-mail(s) tfang@rockefeller.edu
Phone 212-327-8265
Fax 212-327-8258
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (2)
GSE24774 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient DCs)
GSE24826 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response

Data table header descriptions
ID_REF
VALUE percentile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 -0.08100581 0.3183
ILMN_1212612 -0.15648961 0.46115
ILMN_1212619 -0.20277643 0.66541
ILMN_1212628 -0.04509926 1
ILMN_1212632 -0.14078736 0.51378
ILMN_1212636 0.14549828 0.97243
ILMN_1212637 0.2716956 0.76566
ILMN_1212645 2.296409 0.4198
ILMN_1212648 -0.22497177 0.0213
ILMN_1212653 0.07075834 0.06642
ILMN_1212672 0.295846 0.82456
ILMN_1212682 -0.12879801 0.8797
ILMN_1212683 -0.0955317 0.99373
ILMN_1212685 -0.11199689 0.64787
ILMN_1212692 -0.003695488 0.3584
ILMN_1212693 -0.29848027 0.57769
ILMN_1212695 -1.2158108 1
ILMN_1212698 -0.052858353 0.31579
ILMN_1212702 0.84491587 0.5188
ILMN_1212703 0.003071308 1

Total number of rows: 25697

Table truncated, full table size 771 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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