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Sample GSM883429 Query DataSets for GSM883429
Status Public on Mar 02, 2012
Title DC fl 1h pIC Rep1
Sample type RNA
 
Source name G9a f/f DC stimulated with polyI:C for 1h
Organism Mus musculus
Characteristics strain: C57Bl/6
genotype: G9a f/f, Vav-cre
cell type: ex vivo isolated splenic DCs
Treatment protocol DCs were directly stimulated with 10 μg/ml polyI:C in RPMI supplemented with 10% FBS, L-Glutamine, Penicillin-Streptomycin, Sodium Pyruvate, HEPES and 2-mercaptoethanol.
Growth protocol To induce expansion of splenic DCs, C57Bl/6, G9afl/fl mice and G9afl/fl;Vav-Cre mice were subcutaneously injected with 5 x 106 B16-Flt3L cells. Two weeks later splenic DCs were enriched for the CD11c+ population using CD11c MACS beads (Miltenyi).
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp Premier RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description G9a f/f DC stimulated with polyI:C for 1h
Data processing The data were normalised using percentile normalisation with GeneSpringGX 10
 
Submission date Mar 01, 2012
Last update date Mar 02, 2012
Contact name Terry Fang
E-mail(s) tfang@rockefeller.edu
Phone 212-327-8265
Fax 212-327-8258
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (2)
GSE24774 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient DCs)
GSE24826 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response

Data table header descriptions
ID_REF
VALUE percentile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 0.043335676 0.00501
ILMN_1212612 -4.88E-04 0.09774
ILMN_1212619 0.084012985 0.88972
ILMN_1212628 0.076063156 1
ILMN_1212632 0.00319314 0.31328
ILMN_1212636 0.43772697 0.95113
ILMN_1212637 -0.00924921 0.67168
ILMN_1212645 -0.49648833 0.50627
ILMN_1212648 -0.36826324 0.01003
ILMN_1212653 -0.1257329 0.44737
ILMN_1212672 -8.68E-05 0.66165
ILMN_1212682 0.32568645 0.88095
ILMN_1212683 0.022073507 0.92607
ILMN_1212685 -0.101724386 0.20551
ILMN_1212692 -0.051450253 0.10526
ILMN_1212693 -0.03977561 0.65915
ILMN_1212695 0.8207369 0.96366
ILMN_1212698 -0.053128242 0.4599
ILMN_1212702 -0.54021454 0.25439
ILMN_1212703 -0.12112379 1

Total number of rows: 25697

Table truncated, full table size 774 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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