|
| Status |
Public on Mar 02, 2012 |
| Title |
DC ko 1h pIC Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
G9a -/- DC stimulated with polyI:C for 1h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: G9a -/-, Vav-cre
cell type: ex vivo isolated splenic DCs
|
| Treatment protocol |
DCs were directly stimulated with 10 μg/ml polyI:C in RPMI supplemented with 10% FBS, L-Glutamine, Penicillin-Streptomycin, Sodium Pyruvate, HEPES and 2-mercaptoethanol.
|
| Growth protocol |
To induce expansion of splenic DCs, C57Bl/6, G9afl/fl mice and G9afl/fl;Vav-Cre mice were subcutaneously injected with 5 x 106 B16-Flt3L cells. Two weeks later splenic DCs were enriched for the CD11c+ population using CD11c MACS beads (Miltenyi).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp Premier RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
G9a -/- DC stimulated with polyI:C for 1h
|
| Data processing |
The data were normalised using percentile normalisation with GeneSpringGX 10
|
| |
|
| Submission date |
Mar 01, 2012 |
| Last update date |
Mar 02, 2012 |
| Contact name |
Terry Fang |
| E-mail(s) |
tfang@rockefeller.edu
|
| Phone |
212-327-8265
|
| Fax |
212-327-8258
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE24774 |
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient DCs) |
| GSE24826 |
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response |
|
