NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM883436 Query DataSets for GSM883436
Status Public on Mar 02, 2012
Title DC ko 4h pIC Rep2
Sample type RNA
 
Source name G9a -/- DC stimulated with polyI:C for 4h
Organism Mus musculus
Characteristics strain: C57Bl/6
genotype: G9a -/-, Vav-cre
cell type: ex vivo isolated splenic DCs
Treatment protocol DCs were directly stimulated with 10 μg/ml polyI:C in RPMI supplemented with 10% FBS, L-Glutamine, Penicillin-Streptomycin, Sodium Pyruvate, HEPES and 2-mercaptoethanol.
Growth protocol To induce expansion of splenic DCs, C57Bl/6, G9afl/fl mice and G9afl/fl;Vav-Cre mice were subcutaneously injected with 5 x 106 B16-Flt3L cells. Two weeks later splenic DCs were enriched for the CD11c+ population using CD11c MACS beads (Miltenyi).
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp Premier RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description G9a -/- DC stimulated with polyI:C for 4h
Data processing The data were normalised using percentile normalisation with GeneSpringGX 10
 
Submission date Mar 01, 2012
Last update date Mar 02, 2012
Contact name Terry Fang
E-mail(s) tfang@rockefeller.edu
Phone 212-327-8265
Fax 212-327-8258
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (2)
GSE24774 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient DCs)
GSE24826 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response

Data table header descriptions
ID_REF
VALUE percentile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 0.35846305 0.17544
ILMN_1212612 0.46664977 0.14787
ILMN_1212619 0.22498798 0.96617
ILMN_1212628 0.11937189 1
ILMN_1212632 0.322644 0.62907
ILMN_1212636 -0.21319294 0.97243
ILMN_1212637 -0.6033211 0.81078
ILMN_1212645 -0.09331679 0.46992
ILMN_1212648 0.2516222 0.57393
ILMN_1212653 0.3232851 0.38596
ILMN_1212672 1.3895302 0.79574
ILMN_1212682 0.14395285 0.73684
ILMN_1212683 0.39674926 0.99248
ILMN_1212685 0.28719497 0.35338
ILMN_1212692 0.07728577 0.2619
ILMN_1212693 0.22760034 0.80201
ILMN_1212695 0.9615164 0.97744
ILMN_1212698 0.46652126 0.58772
ILMN_1212702 0.042922974 0.73308
ILMN_1212703 0.21011257 1

Total number of rows: 25697

Table truncated, full table size 762 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap