|
| Status |
Public on Dec 19, 2006 |
| Title |
LacZ-5 |
| Sample type |
RNA |
| |
|
| Source name |
LacZ-injected rat left ventricle
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain:Sprague-Dawley rats Gender:male Weight: 250-300 g Tissue:left ventricle
|
| Extracted molecule |
total RNA |
| Label |
Biotin
|
| |
|
| Hybridization protocol |
Wash A1 Recovery Mixes: 0
Wash A1 Temperature (C): 30
Number of Wash A1 Cycles: 10
Mixes per Wash A1 Cycle: 2
Wash B Recovery Mixes: 0
Wash B Temperature (C): 50
Number of Wash B Cycles: 6
Mixes per Wash B Cycle: 15
Stain Temperature (C): 35
First Stain Time (seconds): 300
Wash A2 Recovery Mixes: 0
Wash A2 Temperature (C): 30
Number of Wash A2 Cycles: 10
Mixes per Wash A2 Cycle: 4
Second Stain Time (seconds): 300
Third Stain Time (seconds): 300
Wash A3 Recovery Mixes: 0
|
| Description |
Gene expression profiles three days after MKK3bE + WT p38α gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
|
| Data processing |
GCOS1.2
|
| |
|
| Submission date |
Dec 19, 2005 |
| Last update date |
Dec 30, 2005 |
| Contact name |
Jaana Rysä |
| E-mail(s) |
jaana.rysa@oulu.fi
|
| Phone |
+358-8-5375262
|
| Organization name |
University of Oulu
|
| Department |
Pharmacology and toxicology
|
| Street address |
PO BOX 5000
|
| City |
University of OULU |
| ZIP/Postal code |
90014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE3866 |
p38 MAPK overexpression in heart in vivo. |
|
