|
| Status |
Public on Dec 23, 2005 |
| Title |
Scd1-/- on chow diet replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
Mus musculus (backcrossed to C57BL/6J mice for 5+ generations)
Scd1 knock out mouse, chow diet, liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each individual sample, about 10-50mg flash frozen liver tissue piece was transfeered to -80oC cold RNALater-ICE (Ambion) and placed at -20oC overnight. Tissue was processed according to manufacture's protocol. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 15 ?g of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Scd1 knock out mice, liver, total RNA processed to biotin-labeled cRNA
|
| Data processing |
Affymetrix Microarray Suite version 5.0 was used to generate .CEL files. The .CEL files was processed by Robust Multi-Array Analysis (RMA) in R/Bioconductor to generate expression values represented by RMA
|
| |
|
| Submission date |
Dec 21, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Alan D Attie |
| E-mail(s) |
attie@biochem.wisc.edu
|
| Phone |
608-262-4705
|
| Fax |
608-263-9609
|
| URL |
http://www.biochem.wisc.edu/attie/
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Biochemistry
|
| Lab |
Attie
|
| Street address |
433 Babcock Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE3889 |
Scd1 Knockout Mice on very low fat diet |
|
| Relations |
| Reanalyzed by |
GSE24948 |
| Reanalyzed by |
GSE119085 |
