|
| Status |
Public on Jul 06, 2012 |
| Title |
EB2, embryonic body formation for 2 days with streptozotocin, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
E14 stem cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: EB2, embryonic body
treatment: streptozotocin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared for Illumina arrays per standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: C2
|
| Data processing |
Array data were filtered by detection p-value < 0.05 (similar to signal to noise) in at least 50% samples (we applied a filtering criterion for data analysis; higher signal value was required to obtain a detection p-value < 0.05). Selected gene signal value was transformed by logarithm and normalized by quantile method with IlluminaGUI in R.
|
| |
|
| Submission date |
Mar 07, 2012 |
| Last update date |
Jul 06, 2012 |
| Contact name |
Taewan Kim |
| E-mail(s) |
taewan79@snu.ac.kr
|
| Phone |
082-2-3668-7629
|
| Organization name |
Seoul National University of medicine
|
| Department |
biomedical science
|
| Lab |
metabolic checkpoint lab
|
| Street address |
biomedical science building 202ho
|
| City |
Youngun dong, jongro gu,Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE36322 |
Gene expression analysis of pluripotent and differentiated genes during EB formation treated with streptozotocin, o-glacnacylation inhibitor |
|
