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Sample GSM894459 Query DataSets for GSM894459
Status Public on Mar 14, 2012
Title CD4 memory T cells_72_hour_Young-5
Sample type RNA
 
Source name CD4 memory T cells_72_hour_Young
Organism Homo sapiens
Characteristics cell type: CD4 memory T cells
age: 20-35 years
Treatment protocol T cells were stimulated with myeloid dendritic cells and TSST
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the Rneasy Mini Kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA was obtained using the BioArray HighYield RNA Transcript Labeling Kit (Enzo).
 
Hybridization protocol 10 ug cRNA were hybridized for 16 hr at 45 degree and 60 rpm to Human U133A1 Arrays in a hybridization oven 640 (Affymetrix). GeneChips were washed and stained using the Affymetrix Fluidics450 station.
Scan protocol GeneChips were scanned using a seventh-generation GeneChip Scanner 3000 (Affymetrix).
Description Gene expression data from CD4 memory T cells at 72 hours after stimulation
Data processing The CEL/CHP files were analyzed using Affymetrix default analysis settings and the Rosetta Resolver System.
 
Submission date Mar 13, 2012
Last update date Sep 01, 2016
Contact name Mingcan Yu
Phone (650)723-9027
Fax (650)721-1251
Organization name Stanford University School of Medicine
Department Medicine: Immunology and Rheumatology
Lab Dr. Goronzy lab
Street address CCSR Bldg. Rm. 2215, 269 Campus Drive West
City Stanford
State/province CA
ZIP/Postal code 94305-5166
Country USA
 
Platform ID GPL96
Series (1)
GSE36476 Gene expression in CD4 memory T cell responses with age
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE RMA Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 48.5199 P 1.14058e-10
AFFX-BioB-M_at 429.877 P 1.65249e-30
AFFX-BioB-3_at 1586.57 P 1.62776e-34
AFFX-BioC-5_at 15.9522 P 0.00148318
AFFX-BioC-3_at 81.5299 P 4.07685e-16
AFFX-BioDn-5_at 123.169 P 1.29656e-20
AFFX-BioDn-3_at 161.762 P 2.32051e-23
AFFX-CreX-5_at 199.281 P 2.91987e-25
AFFX-CreX-3_at 27.6638 P 3.55141e-06
AFFX-DapX-5_at 57.3573 P 2.47022e-12
AFFX-DapX-M_at 221.875 P 3.67333e-26
AFFX-DapX-3_at 183.375 P 1.58424e-24
AFFX-LysX-5_at 152.124 P 9.1386e-23
AFFX-LysX-M_at 23.3024 P 3.50315e-05
AFFX-LysX-3_at 39.5408 P 8.07726e-09
AFFX-PheX-5_at 32.7702 P 2.47844e-07
AFFX-PheX-M_at 166.988 P 1.15766e-23
AFFX-PheX-3_at 73.8223 P 5.04011e-15
AFFX-ThrX-5_at 261.948 P 1.91379e-27
AFFX-ThrX-M_at 82.3513 P 3.15968e-16

Total number of rows: 22283

Table truncated, full table size 711 Kbytes.




Supplementary file Size Download File type/resource
GSM894459_20051215_01_Young-5_72_hour.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM894459_20051215_01_Young-5_72hour.CHP.gz 289.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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