NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM896052 Query DataSets for GSM896052
Status Public on May 10, 2012
Title VE-Cadherin+/CD45+(P4) cells from Control Embryo proper(Emb) rep2
Sample type RNA
 
Source name Mouse Embryos (E11.5); Control; Emb; VE-Cadherin+/CD45+(P4) cells
Organism Mus musculus
Characteristics cell type: VE-Cadherin+/CD45+(P4)
strain: mixed (B6/CBA and others)
genotype/variation: Control
tissue: embryo proper
Biomaterial provider Knock-in and Tg mice crossed at CDB RIKEN
Treatment protocol FACS sort only, no treatment afterwards
Growth protocol FACS sort only, no treatment afterwards
Extracted molecule total RNA
Extraction protocol We used Qiagen RNA microelute Kit.
Label Cy3
Label protocol We used Agilent Low Input Quick Amp Labeling Kit, and followed manufacturer's protocol.
http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf
 
Hybridization protocol We used Agilent Hybridization Oven, and followed manufacturer's protocol.
http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf
Scan protocol We used Agilent Microarray Scanner, and followed manufacturer's protocol.
http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf
Data processing We used Agilent Feature Extraction 10.7 for quantification, and GeneSpring GX11.5.1 for data analysis. 1. Data Transformation: Set measurements less than 1 to 1 2. Per Chip: Normalize to 75 percentile 3. Per Gene: Normalize to median
 
Submission date Mar 14, 2012
Last update date May 10, 2012
Contact name Hiroshi Kataoka
Organization name Riken Center for Developmental Biology
Department Stem Cell Biology
Street address 2-2-3 Minatojima-minamimachi, Chuo-ku
City Kobe
State/province Hyogo
ZIP/Postal code 650-0047
Country Japan
 
Platform ID GPL10787
Series (1)
GSE36516 Gene expression profiling in Tie-2-Etv2 endothelial and/or hematopoietic precursors

Data table header descriptions
ID_REF
VALUE Normalized Signal
gFEFlags Flag

Data table
ID_REF VALUE gFEFlags
GE_BrightCorner 1.92E-02 Detected
DarkCorner -0.17428303 Not Detected
A_55_P2051983 -0.030675411 Not Detected
A_52_P169082 -0.60281634 Not Detected
A_30_P01028193 -0.03284216 Not Detected
A_52_P237997 -0.03366661 Not Detected
A_51_P414243 0.90925884 Detected
A_55_P2136348 -0.03577423 Not Detected
A_51_P108228 -0.025780678 Not Detected
A_30_P01033363 -0.02664113 Detected
A_55_P2049737 -0.038708687 Not Detected
A_30_P01024440 -0.012273312 Detected
A_30_P01025554 0.041234493 Detected
A_30_P01031558 -0.14009333 Not Detected
A_30_P01030675 -0.04251194 Not Detected
A_51_P328014 -0.31078148 Detected
A_30_P01019108 -0.3150053 Detected
A_55_P2056220 -0.29493427 Detected
A_55_P1985764 -0.24272394 Detected
A_52_P108321 0.050985098 Detected

Total number of rows: 55821

Table truncated, full table size 1980 Kbytes.




Supplementary file Size Download File type/resource
GSM896052_3_Em_ctl_P4_2_US91403665_252800512468_S02_GE1_107_Sep09_2_2.txt.gz 11.8 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap