|
| Status |
Public on Mar 21, 2012 |
| Title |
Control Diet Supplemented Mouse 5 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 male mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
age: 5 weeks old
gender: male
tissue: B16F10 mouse melanoma cells
diet: control diet
|
| Treatment protocol |
Following a preliminary feeding of each diet formula for one week, 1 × 106 B16F10 cells (suspended with 100 μl of PBS) were subcutaneously injected into the back of the mice. After 2 weeks of diet supplementation, all mice were sacrificed.
|
| Growth protocol |
Five- week- old male C57BL6 mice were randomly divided into two groups and fed control diet (control group, LabDiet, Brentwood, MO, USA) or moderate restriced carbohydrate diet formula (MCD, Treat group) in a specific pathogen free zone. All procedures were approved by the institutional animal use and care committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
control replicate 5
|
| Data processing |
Raw data were extracted using the Illumina GenomeStudio. Array data were filtered by detection p-value < 0.05 (similar to signal to noise) in at least 50% samples (we applied a filtering criterion for data analysis; higher signal value was required to obtain a detection p-value < 0.05). Selected gene signal value was divided by Actb signal value.
AVG signal divided by Actb AVG signal
|
| |
|
| Submission date |
Mar 20, 2012 |
| Last update date |
Mar 26, 2012 |
| Contact name |
Jong-Doo Lee |
| E-mail(s) |
JDLEE@yuhs.ac
|
| Phone |
82-10-2829-1159
|
| Fax |
82-2-312-0578
|
| Organization name |
Yonsei University
|
| Street address |
250 seonsan-ro seodaemum-gu
|
| City |
Seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE36633 |
Analysis of differential genetic expression by dietary intervention using moderate-carbohydrate restriction diet supplemented with calorie restriction mimetic multiple phytochemicals extracts(MCDmp) in B16F10 xenografted mouse model. |
|
