|
| Status |
Public on Mar 22, 2012 |
| Title |
10 uM 6h, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
SK-N-SH cells treated with Risperidone 10uM for 6h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SK-N-SH
treatment: treated with Risperidone 10uM for 6h
|
| Treatment protocol |
the cells were incubated for increasing times, up to 48 h, with 0.1, 1 and 10 µM risperidone. Gene expression was assessed by microarray in cells treated with risperidone 10 µM at 6, 24 and 48 h. Each condition was assessed in quadruplicate. Risperidone was resuspended in DMSO. In each experiment, controls were treated with the vehicle (DMSO) only. The vehicle did not exhibit cytotoxic effects at the concentrations used in the experiments (maximum concentration 1% of total volume).
|
| Growth protocol |
SK-N-SH cell line was grown at 37ºC in a humidified incubator with 5% of CO2 in MEM supplemented with 10% FBS, 2 mM L-glutamine, sodium pyruvate, 50 U/mL penicillin and 50 µg/mL streptomycin. Cells were seeded at 3x105 cells/well in 6-well culture plates. The cells were allowed to grow for 48 h to reach 95% confluence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
|
| |
|
| Hybridization protocol |
Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
|
| Scan protocol |
Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
|
| Data processing |
RMA processing algorithm. Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
|
| |
|
| Submission date |
Mar 21, 2012 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Sergi Mas Herrero |
| E-mail(s) |
sergimash@ub.edu
|
| Phone |
+34 934024526
|
| Organization name |
University of Barcelona
|
| Department |
Pharmacology, School of Medicine
|
| Street address |
Casanova 143
|
| City |
Barcelona |
| State/province |
Catalunya |
| ZIP/Postal code |
08030 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE36678 |
Expression data SK-N-SH cells treated with risperidone |
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