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Sample GSM898698 Query DataSets for GSM898698
Status Public on Dec 31, 2012
Title MCF7_control72h_EtOh6h_4
Sample type RNA
 
Source name MCF7 cells, 72h no treatment, 6h EtOh treatment, rep 4
Organism Homo sapiens
Characteristics cell line: MCF7 breast cancer cells
dac treatment: 72h no treatment
post-dac treatment: 6h EtOH treatment
Treatment protocol During these 72h, the cells were either not treated or treated with 1μM DAC (Sigma Aldrich). Culture medium with or without treatment was changed every 24h. The DAC-treated cells were subsequently treated with vehicle (ethanol, EtOh), and the non-treated cells were subsequently treated with either vehicle or 10nM E2 for 6h. Each treatment was done in four replicates.
Growth protocol MCF7 cells were seeded in plates and cultured in phenol red-free DMEM supplemented with 2.5% dextran-coated charcoal-treated heat-inactivated FBS (Hyclone) for 72 h.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen)
Label biotin
Label protocol GeneChip 3' IVT Express kit starting with 200 ng of total RNA.
 
Hybridization protocol Array plates were washed and stained in the Affymetrix Gene Titan system.
Scan protocol Array plates were scanned according to Affymetrix Gene Titan standard procedure.
Description Sample name: MP4
C_EtOH
Data processing Summarization: RMA, Normalization: Sketch-Quantile, Transformation: to linear scale
Software: Affymetrix Expression Console version 1.1
 
Submission date Mar 21, 2012
Last update date Dec 31, 2012
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
 
Platform ID GPL13158
Series (1)
GSE36683 Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
203874_PM_s_at 51.62507259
229475_PM_at 7.568461174
226281_PM_at 12.29500145
201666_PM_at 282.0877097
204780_PM_s_at 47.17661495
207113_PM_s_at 23.26356028
200665_PM_s_at 11.39240156
221875_PM_x_at 134.3637355
239430_PM_at 1698.44643
209140_PM_x_at 393.4400464
210074_PM_at 101.8286699
216526_PM_x_at 604.6682427
218856_PM_at 182.278425
208949_PM_s_at 734.185087
231265_PM_at 11.39240156
227449_PM_at 36.00187151
227671_PM_at 7.889861636
210105_PM_s_at 20.2521055
223454_PM_at 165.4211623
225352_PM_at 630.3459396

Total number of rows: 54715

Table truncated, full table size 1366 Kbytes.




Supplementary file Size Download File type/resource
GSM898698_HT_HG-U133_Plus_PM_F09_MP4.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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