NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM898700 Query DataSets for GSM898700
Status Public on Dec 31, 2012
Title MCF7_control72h_E26h_2
Sample type RNA
 
Source name MCF7 cells, 72h no treatment, 6h E2 treatment, rep 2
Organism Homo sapiens
Characteristics cell line: MCF7 breast cancer cells
dac treatment: 72h no treatment
post-dac treatment: 6h E2 treatment
Treatment protocol During these 72h, the cells were either not treated or treated with 1μM DAC (Sigma Aldrich). Culture medium with or without treatment was changed every 24h. The DAC-treated cells were subsequently treated with vehicle (ethanol, EtOh), and the non-treated cells were subsequently treated with either vehicle or 10nM E2 for 6h. Each treatment was done in four replicates.
Growth protocol MCF7 cells were seeded in plates and cultured in phenol red-free DMEM supplemented with 2.5% dextran-coated charcoal-treated heat-inactivated FBS (Hyclone) for 72 h.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen)
Label biotin
Label protocol GeneChip 3' IVT Express kit starting with 200 ng of total RNA.
 
Hybridization protocol Array plates were washed and stained in the Affymetrix Gene Titan system.
Scan protocol Array plates were scanned according to Affymetrix Gene Titan standard procedure.
Description Sample name: MP6
C_E2
Data processing Summarization: RMA, Normalization: Sketch-Quantile, Transformation: to linear scale
Software: Affymetrix Expression Console version 1.1
 
Submission date Mar 21, 2012
Last update date Dec 31, 2012
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
 
Platform ID GPL13158
Series (1)
GSE36683 Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
203874_PM_s_at 50.91433496
229475_PM_at 7.012845771
226281_PM_at 11.3137085
201666_PM_at 232.3249038
204780_PM_s_at 69.07060714
207113_PM_s_at 32.2225776
200665_PM_s_at 8.876555777
221875_PM_x_at 139.1020624
239430_PM_at 1323.369299
209140_PM_x_at 388.023441
210074_PM_at 78.24897777
216526_PM_x_at 568.0995697
218856_PM_at 407.3146797
208949_PM_s_at 754.8258393
231265_PM_at 11.23555901
227449_PM_at 36.50443891
227671_PM_at 8.0556444
210105_PM_s_at 29.65081798
223454_PM_at 207.9366135
225352_PM_at 694.5814157

Total number of rows: 54715

Table truncated, full table size 1366 Kbytes.




Supplementary file Size Download File type/resource
GSM898700_HT_HG-U133_Plus_PM_E05_MP6.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap