NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM898704 Query DataSets for GSM898704
Status Public on Dec 31, 2012
Title MCF7_DAC72h_EtOh6h_2
Sample type RNA
 
Source name MCF7 cells, 72h 1μM DAC treatment, 6h EtOh treatment, rep 2
Organism Homo sapiens
Characteristics cell line: MCF7 breast cancer cells
dac treatment: 72h 1μM DAC treatment
post-dac treatment: 6h EtOH treatment
Treatment protocol During these 72h, the cells were either not treated or treated with 1μM DAC (Sigma Aldrich). Culture medium with or without treatment was changed every 24h. The DAC-treated cells were subsequently treated with vehicle (ethanol, EtOh), and the non-treated cells were subsequently treated with either vehicle or 10nM E2 for 6h. Each treatment was done in four replicates.
Growth protocol MCF7 cells were seeded in plates and cultured in phenol red-free DMEM supplemented with 2.5% dextran-coated charcoal-treated heat-inactivated FBS (Hyclone) for 72 h.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen)
Label biotin
Label protocol GeneChip 3' IVT Express kit starting with 200 ng of total RNA.
 
Hybridization protocol Array plates were washed and stained in the Affymetrix Gene Titan system.
Scan protocol Array plates were scanned according to Affymetrix Gene Titan standard procedure.
Description Sample name: MP10
A_EtOH
Data processing Summarization: RMA, Normalization: Sketch-Quantile, Transformation: to linear scale
Software: Affymetrix Expression Console version 1.1
 
Submission date Mar 21, 2012
Last update date Dec 31, 2012
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
 
Platform ID GPL13158
Series (1)
GSE36683 Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
203874_PM_s_at 215.2694823
229475_PM_at 240.5178238
226281_PM_at 73.00887782
201666_PM_at 576.0299442
204780_PM_s_at 298.1717981
207113_PM_s_at 164.2785149
200665_PM_s_at 107.6347412
221875_PM_x_at 501.4631924
239430_PM_at 4837.345941
209140_PM_x_at 1379.567183
210074_PM_at 380.0380341
216526_PM_x_at 1734.1344
218856_PM_at 413.0005807
208949_PM_s_at 2320.146197
231265_PM_at 44.94223602
227449_PM_at 324.0336881
227671_PM_at 28.8400148
210105_PM_s_at 95.67035191
223454_PM_at 421.6786008
225352_PM_at 504.9511447

Total number of rows: 54715

Table truncated, full table size 1366 Kbytes.




Supplementary file Size Download File type/resource
GSM898704_HT_HG-U133_Plus_PM_D07_MP10.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap