|
| Status |
Public on Dec 31, 2012 |
| Title |
MCF7_DAC72h_EtOh6h_3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cells, 72h 1μM DAC treatment, 6h EtOh treatment, rep 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7 breast cancer cells
dac treatment: 72h 1μM DAC treatment
post-dac treatment: 6h EtOH treatment
|
| Treatment protocol |
During these 72h, the cells were either not treated or treated with 1μM DAC (Sigma Aldrich). Culture medium with or without treatment was changed every 24h. The DAC-treated cells were subsequently treated with vehicle (ethanol, EtOh), and the non-treated cells were subsequently treated with either vehicle or 10nM E2 for 6h. Each treatment was done in four replicates.
|
| Growth protocol |
MCF7 cells were seeded in plates and cultured in phenol red-free DMEM supplemented with 2.5% dextran-coated charcoal-treated heat-inactivated FBS (Hyclone) for 72 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
GeneChip 3' IVT Express kit starting with 200 ng of total RNA.
|
| |
|
| Hybridization protocol |
Array plates were washed and stained in the Affymetrix Gene Titan system.
|
| Scan protocol |
Array plates were scanned according to Affymetrix Gene Titan standard procedure.
|
| Description |
Sample name: MP11
A_EtOH
|
| Data processing |
Summarization: RMA, Normalization: Sketch-Quantile, Transformation: to linear scale
Software: Affymetrix Expression Console version 1.1
|
| |
|
| Submission date |
Mar 21, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Milica Putnik |
| Organization name |
Karolinska Institute
|
| Department |
Biosciences and Nutrition
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE36683 |
Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells |
|
