|
Status |
Public on Sep 01, 2012 |
Title |
3_nebulin_+/- [miRNA] |
Sample type |
RNA |
|
|
Source name |
mouse HZ3 heterozygous
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: Nebulin +/- tissue: quadriceps
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl).
|
Label |
Biotin
|
Label protocol |
Samples were labeled using the FlashTag™ Biotin HSR Kit from Genisphere starting with 1µg of totel RNA according to the protocol of the manufaturer
|
|
|
Hybridization protocol |
according to the recommended protocol followed by washing at a Fluidics Station 450
|
Scan protocol |
Affymetrix GeneArray Scanner3000
|
Description |
quadriceps total RNA of mouse HZ3 with one nebulin allele
|
Data processing |
Data were processed using the miRNAQC tool from Affymetrix following the recommended workflow from Genisphere (Background Detection -> RMA Global Background Correction -> Quantile Normalization -> Median polish Summarization); Statistical analysis was done using SAS JMP Genomics 4.0
|
|
|
Submission date |
Mar 23, 2012 |
Last update date |
Sep 01, 2012 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
|
Department |
ZMF
|
Street address |
Theodor-Kutzer-Ufer
|
City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
|
|
Platform ID |
GPL8786 |
Series (2) |
GSE36741 |
In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [miRNA] |
GSE36745 |
In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy |
|