|
| Status |
Public on Sep 01, 2012 |
| Title |
3_nebulin_+/+ [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
mouse WT3 wild type
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: nebulin +/+
tissue: quadriceps
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl).
|
| Label |
Biotin
|
| Label protocol |
Samples were labeled using the FlashTag™ Biotin HSR Kit from Genisphere starting with 1µg of totel RNA according to the protocol of the manufaturer
|
| |
|
| Hybridization protocol |
according to the recommended protocol followed by washing at a Fluidics Station 450
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
quadriceps total RNA of mouse WT3 with both nebulin alleles
|
| Data processing |
Data were processed using the miRNAQC tool from Affymetrix following the recommended workflow from Genisphere (Background Detection -> RMA Global Background Correction -> Quantile Normalization -> Median polish Summarization); Statistical analysis was done using SAS JMP Genomics 4.0
|
| |
|
| Submission date |
Mar 23, 2012 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE36741 |
In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [miRNA] |
| GSE36745 |
In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy |
|
