NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM900089 Query DataSets for GSM900089
Status Public on Sep 01, 2012
Title 3_nebulin_+/+ [miRNA]
Sample type RNA
 
Source name mouse WT3 wild type
Organism Mus musculus
Characteristics genotype/variation: nebulin +/+
tissue: quadriceps
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol total RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl).
Label Biotin
Label protocol Samples were labeled using the FlashTag™ Biotin HSR Kit from Genisphere starting with 1µg of totel RNA according to the protocol of the manufaturer
 
Hybridization protocol according to the recommended protocol followed by washing at a Fluidics Station 450
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps total RNA of mouse WT3 with both nebulin alleles
Data processing Data were processed using the miRNAQC tool from Affymetrix following the recommended workflow from Genisphere (Background Detection -> RMA Global Background Correction -> Quantile Normalization -> Median polish Summarization); Statistical analysis was done using SAS JMP Genomics 4.0
 
Submission date Mar 23, 2012
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL8786
Series (2)
GSE36741 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [miRNA]
GSE36745 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 1.162701
14qI-1_st 1.502732
14qI-2_st 0.7521954
14qI-3_x_st 1.444671
14qI-4_st 1.185023
14qI-4_x_st 1.751027
14qI-5_st 1.196523
14qI-6_st 0.8491195
14qI-6_x_st 1.560384
14qI-7_st 1.40575
14qI-8_st 1.365277
14qI-8_x_st 1.254547
14qI-9_x_st 1.373625
14qII-10_st 1.222761
14qII-11_st 1.145644
14qII-11_x_st 0.8579944
14qII-12_st 0.9477493
14qII-12_x_st 1.155969
14qII-13_st 1.222761
14qII-14_st 1.067963

Total number of rows: 7815

Table truncated, full table size 190 Kbytes.




Supplementary file Size Download File type/resource
GSM900089_Witt_240811_WT3_miRNA-1_0_2Xgain_.CEL.gz 156.5 Kb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap