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Sample GSM900103 Query DataSets for GSM900103
Status Public on Sep 01, 2012
Title 2_nebulin_+/- [mRNA]
Sample type RNA
Source name mouse HZ2 heterozygous
Organism Mus musculus
Characteristics genotype/variation: nebulin +/-
tissue: quadriceps
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse HZ2 with one nebulin allele
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
Submission date Mar 23, 2012
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platform ID GPL14661
Series (2)
GSE36743 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [mRNA]
GSE36745 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy

Data table header descriptions
VALUE RMA signal intensity

Data table
AFFX-BioB-5_at 8.587118
AFFX-BioB-M_at 8.893188
AFFX-BioB-3_at 8.732968
AFFX-BioC-5_at 9.568502
AFFX-BioC-3_at 9.876706
AFFX-BioDn-5_at 11.02339
AFFX-BioDn-3_at 11.84283
AFFX-CreX-5_at 13.02965
AFFX-CreX-3_at 12.91719
AFFX-DapX-5_at 8.906318
AFFX-DapX-M_at 10.04457
AFFX-DapX-3_at 10.1588
AFFX-LysX-5_at 6.874531
AFFX-LysX-M_at 7.179367
AFFX-LysX-3_at 7.591144
AFFX-PheX-5_at 7.396466
AFFX-PheX-M_at 7.444832
AFFX-PheX-3_at 7.781254
AFFX-ThrX-5_at 7.86992
AFFX-ThrX-M_at 7.801254

Total number of rows: 16556

Table truncated, full table size 295 Kbytes.

Supplementary file Size Download File type/resource
GSM900103_Witt_150311_HZ_2_Mouse430_2_.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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