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Sample GSM900104 Query DataSets for GSM900104
Status Public on Sep 01, 2012
Title 3_nebulin_+/- [mRNA]
Sample type RNA
Source name mouse HZ3 heterozygous
Organism Mus musculus
Characteristics genotype/variation: nebulin +/-
tissue: quadriceps
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse HZ3 with one nebulin allele
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
Submission date Mar 23, 2012
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platform ID GPL14661
Series (2)
GSE36743 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [mRNA]
GSE36745 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy

Data table header descriptions
VALUE RMA signal intensity

Data table
AFFX-BioB-5_at 8.472028
AFFX-BioB-M_at 8.787582
AFFX-BioB-3_at 8.632197
AFFX-BioC-5_at 9.432104
AFFX-BioC-3_at 9.716925
AFFX-BioDn-5_at 10.9138
AFFX-BioDn-3_at 11.76591
AFFX-CreX-5_at 13.00283
AFFX-CreX-3_at 12.90827
AFFX-DapX-5_at 8.780545
AFFX-DapX-M_at 9.8185
AFFX-DapX-3_at 9.981567
AFFX-LysX-5_at 6.629631
AFFX-LysX-M_at 7.014294
AFFX-LysX-3_at 7.442374
AFFX-PheX-5_at 7.208458
AFFX-PheX-M_at 7.244294
AFFX-PheX-3_at 7.589753
AFFX-ThrX-5_at 7.590876
AFFX-ThrX-M_at 7.591987

Total number of rows: 16556

Table truncated, full table size 295 Kbytes.

Supplementary file Size Download File type/resource
GSM900104_Witt_150311_HZ_3_Mouse430_2_.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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